2-Oxoglutaric Acid → cis-3-Hydroxy-L-proline

HRID 848960

Reaktionsgleichung

Reaktionsgleichung von HRID 848960
[CH2:1]([NH:7][C:8]([CH2:13][OH:14])([CH2:11][OH:12])CO)[CH2:2]S(O)(=O)=O.N1CCC[C@H]1C(O)=[O:18].O=C(CCC(O)=O)C(O)=O.O=C1O[C@H]([C@H](CO)O)C(O)=C1O>>[OH:14][C@@H:13]1[CH2:2][CH2:1][NH:7][C@@H:8]1[C:11]([OH:12])=[O:18]

Komponenten

5

Edukt 4

HCID 512-Oxoglutaric AcidHCID 81831N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acidHCID 145742L-ProlineHCID 54670067L-Ascorbic Acid

Produkt 1

HCID 7098652cis-3-Hydroxy-L-proline

Reaktionsbedingungen

Temperatur
100 °C

Versuchsvorschrift

The cells, the treated cells or the enzyme preparation are added to 240 mM TES [N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid] buffer containing 12 mM L-proline, 24 mM 2-ketoglutaric acid, 4 mM ferrous sulfate and 8 mM L-ascorbic acid to make 250 μl in total. The mixture is kept at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes to stop the reaction, and the amount of cis-3-hydroxy-L-proline produced in the reaction mixture is determined by HPLC.

Aufarbeitung

  1. customThe cells, the treated cells or the enzyme preparation
  2. customthe reaction

Quellen und Nachweise

ORD-Eintrag
ord-2fc0112d8fc54d6b959e8678c3b53275
Quelldatensatz
uspto-grants-2000_01
Patentnummer
US06010891

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