Leukotriene C4 → Leukotrien C4

HRID 851833

Reaktionsgleichung

Reaktionsgleichung von HRID 851833
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5]/[CH:6]=[CH:7]\[CH2:8]/[CH:9]=[CH:10]\[CH:11]=[CH:12]\[CH:13]=[CH:14]\[C@@H:15]([S:24][CH2:25][C@H:26]([NH:34][C:35]([CH2:37][CH2:38][C@H:39]([NH2:43])[C:40]([OH:42])=[O:41])=[O:36])[C:27]([NH:29][CH2:30][C:31]([OH:33])=[O:32])=[O:28])[C@@H:16]([OH:23])[CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21].CCCCC/C=C\C/C=C\C=C\C=C\[C@@H]1O[C@H]1CCCC(OC)=O.B(OC[C@@H](C(O)=O)N)(O)O>P([O-])([O-])([O-])=O>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5]/[CH:6]=[CH:7]\[CH2:8]/[CH:9]=[CH:10]\[CH:11]=[CH:12]\[CH:13]=[CH:14]\[C@@H:15]([S:24][CH2:25][C@@H:26]([NH:34][C:35]([CH2:37][CH2:38][C@H:39]([NH2:43])[C:40]([OH:42])=[O:41])=[O:36])[C:27]([NH:29][CH2:30][C:31]([OH:33])=[O:32])=[O:28])[C@@H:16]([OH:23])[CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21]

Komponenten

5

Edukt 3

HCID 5280493Leukotriene C4HCID 6478221methyl 4-[(2S,3S)-3-[(1E,3E,5Z,8Z)-tetradeca-1,3,5,8-tetraenyl]oxiran-2-yl]butanoateHCID 18648594Serine borate

Produkt 1

HCID 44563898Leukotrien C4

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 1061PhosphateLösungsmittel

Versuchsvorschrift

LTC4 synthase prepared as a crude fraction from guinea pig lung was used. The test compounds7were tested in duplicate at a concentration of 300 μg/ml. The test compound and/or vehicle was incubated with 12 μg enzyme, 0.3 μg LTA4 methyl ester, 0.2% (w/w) albumin (to stabilize the product) and 4.5 mM serine borate (to prevent conversion of LTC4 to LTD4) in phosphate buffer pH 7.8 for 30 minutes at 37° C. The reaction was terminated by addition of ice-cold methanol. Formation of LTC4 was quantitated by RIA (radioimmunoassay).

Aufarbeitung

  1. customfor 30 minutes
  2. customat 37° C
  3. customThe reaction was terminated by addition of ice-cold methanol

Quellen und Nachweise

ORD-Eintrag
ord-1cfbfd07b15548a49aa5a01e196e25f6
Quelldatensatz
uspto-grants-2007_08
Patentnummer
US07252845B2

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