NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

Beteiligte Stoffe

Reaktionsschritte

The concentration of α-glucose 1-phosphate is determined according to the following procedure: 17.1 mg of Na2EDTA is dissolved in 47 mM potassium phosphate buffer (pH 7.0) to prepare 100 ml of a solution. A mixture of 2.5 ml of the solution, 100 μl of a 14.8 mM NADP+ solution, 100 μl of a 26 mM magnesium chloride solution, 100 μl of a 1.34 mM glucose 1,6-diphosphate solution, 50 μl of a 31 unit/ml phosphoglucomutase solution, 50 μl of a 35 unit/ml glucose 6-phosphate dehydrogenase solution, and 100 μl of a sample solution is incubated at 30° C. for 30 min. and subjected to spectrophotometric measurement at 340 nm to give the amount of produced NADPH, thus determining indirectly the amount of α-glucose 1-phosphate in the sample solution.

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