Resazurin

HCID 11077CAS 550-82-3

Substructure search Find similar structures

Basic information

Standard SMILES: O=c1ccc2[n+]([O-])c3ccc(O)cc3oc-2c1
InChIKey: PLXBWHJQWKZRKG-UHFFFAOYSA-N
Molecular formula: C12H7NO4
Average molar mass: 229.19 g/mol
Monoisotopic mass: 229.0375077

Overview

Resazurin is a phenoxazine.

Names & identifiers

Preferred name: Resazurin
IUPAC: 7-hydroxy-10-oxidophenoxazin-10-ium-3-one
English name: RESAZURIN
Molecular formula: C12H7NO4
Molecular weight: 229.19

Names & synonyms

RESAZURIN550-82-3DiazoresorcinolAzoresorcinResazoinResazurine7-Hydroxy-3H-phenoxazin-3-one 10-oxide3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxideRHODALUXDTXSID40602801FN9YD6968NSC-10438

Show more names

Nsc 56323Nsc 10438DiazoresorcinalResazurin tablets7-HYDROXY-10-OXY-PHENOXAZIN-3-ONE7-hydroxy-3h-fenoxazin-3-on-10-oxid7-hydroxy-3h-phenoxazin-3-on10-oxide

NSC-563237-hydroxy-10-oxido-phenoxazin-10-ium-3-onereazurinreazurineAlamarblueRefChem:178890DTXCID3041823208-987-1

Database identifiers

CAS: 550-82-3
PubChem CID: 11077
DTXSID: DTXSID4060280
ChEMBL: CHEMBL1616414
ChEBI: CHEBI:8806
UNII: 1FN9YD6968
EC Number: 208-987-1
Nikkaji: J13.744H

Properties

Computed structural properties

XLogP: -0.1
Topological polar surface area: 75.3 Å²
Hydrogen bond donors: 1
Hydrogen bond acceptors: 4
Rotatable bonds: 0
Heavy atoms: 17
Formal charge: 0
Complexity: 463

Experimental & physicochemical properties

Chemical ClassesDyes -> Azine Dyes

Physical DescriptionDark red solid with a greenish luster; [Merck Index] Red odorless crystals; [Carolina Biological Supply MSDS]

Physicochemical properties

Melting point: 250 °C · >250°C
Boiling point: 477.2 · 477.2±55.0 °C(Predicted)
Density: 1.57 · 1.57±0.1 g/cm3(Predicted)
solubility: Solubility Insoluble in water, ether; sparingly soluble in ethanol
pka: 6.71 · 6.71(at 25℃)
color: Dark red crystals with greenish luster
PH Range: Orange (3.8) to purple-violet (6.5)
λmax: 598 nm, · 598nm, 380nm
Major Application: Display device, inks, detergents, analysis of soil samples, cosmetics, measuring freshness of fruit, assays for suring freshness of fruit, sorbitol, monitoring sugar loss, assessment of microbials, assays for cells, mitrochondria, semen quality, detecting antiviral substances, diagnosis of bacterialand fungal infection, treating pulmonary tuberculosis, oral care formulation
FDA UNII: 1 FN9YD6968 · 1FN9YD6968
EPA Substance Registry System: Resazurin (550-82-3)
Symbol(GHS): 7 · GHS07
Signal word: Warning
Hazard statements: 335 · H335-H319-H315
Precautionary statements: 264 · P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P
TSCA: TSCA listed

Safety & regulation

GHS classification

GHS Classification · 5 items

This chemical does not meet GHS hazard criteria for 7.3% (3 of 41) of reports.

Warning

H315 (92.7%): Causes skin irritation [Warning Skin corrosion/irritation]

P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)

Aggregated GHS information provided per 41 reports by companies from 2 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.

Hazard information

Regulatory Information · 2 items

Chemical: 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide

3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide: Does not have an individual approval but may be used as a component in a product covered by a group standard. It is not approved for use as a chemical in its own right.

Hazards SummaryA skin irritant; Harmful by ingestion; [Carolina Biological Supply MSDS]

Hazard Classes and CategoriesSkin Irrit. 2 (92.7%)

Safety data

tsca: TSCA listed
Signal word: Warning
Symbol(GHS): GHS07
Hazard statements: H335-H319-H315
Precautionary statements: P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P

Chemistry & industry

Pharmacology

MeSH Pharmacological Classification · 1 item

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)

Uses & manufacturing

UsesUsed as a pH indicator, to detect hyposulfite, and in food research (reductase test); [Merck Index]

General Manufacturing Information3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide: ACTIVE

Applications

DescriptionResazurin is a blue dye used as an oxidation-reduction (redox) indicator in various biochemical and cell viability assays. It is particularly valued for its ability to measure cell metabolic activity.

UsesAs indicator, using a solution of 0.1 g in 20 ml 0.1N NaOH, and water to make 500 ml. pH: 3.8 orange, 6.5 dark violet. In the detection of hyposulfite (sulfoxylate). In food research (reductase test).