2-Cyanoethyl tetraisopropylphosphorodiamidite

Description2-Cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite is a colorless viscous liquid, which is soluble in most organic solvents. It is a widely used phosphitylating reagent for the preparation of various phosphorylated biomolecules, such as nucleoside carbohydrate conjugates, phospholipids and glycopeptides. In particular, this reagent is highly effective for automated solid-phase DNA/RNA oligonucleotide synthesis. It has shown great utility in the coupling of nucleobases or carbohydrates via their phosphotriesters in the presence of activators such as 1H-tetrazole, in moderate yields under mild conditions.This compound is cheaper and more stable than 2-cyanoethyl N,N-diisopropylchlorophosphorodiamidite (the other commonly used phosphinylating reagent)[1].

UsesReagent for synthesizing phosphitylated nucleotides.1

Uses2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphordiamidite was used:in preparation of prosphoramidite reagent, required for synthesis of 12-mer oligodeoxynucleotideas phosphorylating agent in synthesis of 1,2-diacyl-sn-glycerophosphatidylserinein situ preparation of deoxyribonucleoside phosphoramiditesin preparation of 2′-deoxy-2′-fluoro-3′-O-(β-cyanoethyl-N,N-diisopropylphosphoramidic)-5′-O-(4-methoxytrityl)-4′-thio-β-D-arabinouridine and 1-(3-O-(β-cyanoethyl-N,N-diisopropylphosphoramidic)-2-deoxy-2-fluoro-5-O-(4,4′-dimethoxytrityl)-4-thio-β-D-arabinofuranosyl)-thymineas reagent for synthesizing phosphitylated nucleotides