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Giemsa Stain

HCID 13735
NotesReactions
Basic informationOverviewNames & identifiersPropertiesSafety & regulationChemistry & industryRelated reactionsCompound notes
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Basic information

CAS
51811-82-6
Standard SMILES
C[N+](C)=c1ccc2nc3ccc(N)cc3sc-2c1.[Cl-]
InChIKey
NALREUIWICQLPS-UHFFFAOYSA-N
Molecular formula
C14H14ClN3S
Average molar mass
291.8 g/mol
Monoisotopic mass
291.0596963

Overview

Azure A is an organic chloride salt having 3-amino-7-(dimethylamino)phenothiazin-5-ium as the counterion. It is used in making azure eosin stains for blood smear staining. It has a role as a fluorochrome and a histological dye. It contains a 3-amino-7-(dimethylamino)phenothiazin-5-ium.

Names & identifiers

Preferred name
Giemsa Stain
IUPAC
(7-aminophenothiazin-3-ylidene)-dimethylazanium chloride
English name
AZURE A EOSINATE, PURE
Molecular formula
C34H20Br4N3O5S-
Molecular weight
902.23

Names & synonyms

Giemsa stain51811-82-6Giemsa's stainStain, GiemsaRefChem:922205257-438-2AZURE A531-53-3DimethylthionineAzure A chloride3-Amino-7-(dimethylamino)phenothiazin-5-ium chlorideAzur A
Show more names
AZURE A EOSINATE, PURE3-amino-7-(dimethylamino)phenothiazin-5-ium, salt with 2',4',5',7'-tetrabromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one (2:1)
N,N-DimethylthionineC.I. 52005Phenothiazin-5-ium, 3-amino-7-(dimethylamino)-, chloride3-Amino-7-dimethylaminophenothiazin-5-ium chlorideasym-Dimethyl-3,7-diaminophenazathionium chloride3-Amino-7-dimethylaminophenazathionium chlorideAzure A (chloride)Azure A (Technical Grade)

Database identifiers

CAS
51811-82-6、531-53-3、62298-43-5、8050-34-8
PubChem CID
13735
DTXSID
DTXSID2040157
ChEMBL
CHEMBL3330735
ChEBI
CHEBI:87637
UNII

Properties

Computed structural properties

Topological polar surface area
66.7 Ų
Hydrogen bond donors
1
Hydrogen bond acceptors
4
Rotatable bonds
0
Heavy atoms
19
Formal charge
0
Complexity
483

Experimental & physicochemical properties

Chemical ClassesDyes -> Azine Dyes
Density0.89 @25 °C
Melting Point300 °C
Physical DescriptionDark green crystalline powder; [Acros Organics MSDS]

Physicochemical properties

storage temp.
room temp
form
powder or crystals
color
green to black
λmax
522 nm · 522 nm (2nd)628 nm
Major Application
diagnostic assay manufacturinghematologyhistology
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChIKey
NALREUIWICQLPS-UHFFFAOYSA-N
SMILES
[Cl-].C\[N+](C)=C1/C=CC2=Nc3ccc(N)cc3SC2=C1
CAS DataBase Reference
62298 · 62298-43-5

Safety & regulation

Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.

GHS classification

GHS Classification · 4 items

Warning

H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation]

P264+P265, P280, P305+P351+P338, and P337+P317 (click each P-code to see the statement)

Aggregated GHS information provided per 6 reports by companies from 2 notifications to the ECHA C&L Inventory.

Hazard information

Regulatory Information · 2 items

Chemical: Giemsa's stain

Giemsa's stain: Does not have an individual approval but may be used under an appropriate group standard

Hazards SummaryMay cause irritation; [Acros Organics MSDS]
Hazard Classes and CategoriesEye Irrit. 2 (100%)

Safety & protection

Protective Action Criteria (PAC) · 3 items

0.23 [mg/m3]

2.5 [mg/m3]

15 [mg/m3]

Safety data

wgk
3
hs_code
32041900
Storage Class
11 - Combustible Solids

Chemistry & industry

Pharmacology

MeSH Pharmacological ClassificationChemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.

Uses & manufacturing

General Manufacturing InformationGiemsa's stain: ACTIVE

Applications

Usesdiagnostic assay manufacturinghematologyhistology

Suppliers100 total

北京华美互利生物化工010-56205725 · [email protected]
阿诺西(上海)生物技术有限公司400-1107768 15058188853 · [email protected]
上海阿拉丁生化科技股份有限公司400-6206333 13167063860 · [email protected]
湖南韵邦生物科技股份有限公司0731-88532326 13319508186 · [email protected]
湖北威德利化学试剂有限公司186-02735115 18062128043 · [email protected]
武汉英诺医药科技有限公司+86-027-59232304 15387063101 · [email protected]
上海康朗生物科技有限公司021-61998208 18217752821 · [email protected]
艾吉析科技(上海)有限公司17717235263 · [email protected]

Related reactions

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Compound notes

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On this pageBasic informationOverviewNames & identifiersPropertiesSafety & regulationChemistry & industryRelated reactionsCompound notes
M731V243EF
EC Number
257-438-2
UNSPSC Code
12171500 · 12171500
NACRES
47 · NA.47
WGK Germany
3 · 3
HS Code
32041900 · 32041900
Storage Class
11 · 11 - Combustible Solids
郑州汇聚化工有限公司0371-53399770 18037166334 · [email protected]
广州纳珑化工有限公司13192879270; 13192879270 · [email protected]
上海哲研生物科技有限公司021-18017610038 18017610038 · [email protected]
广州佳途科技股份有限公司81960175-877 18933936954 · [email protected]
咸宁深蓝生物医药研发有限公司18171815831 · [email protected]
杭州品高化工有限公司0571-87658025 · [email protected]
湖北科乐精细化工有限公司027-59101668 19945030958 · [email protected]
上海弘顺生物科技有限公司15901914505 · [email protected]
上海晶抗生物工程有限公司021-54721350 13524668266 · [email protected]
济南启源生物科技有限公司18766124282; 18766124282 · [email protected]
安耐吉化学&3A(安徽泽升科技有限公司)021-58432009 400-005-6266 · [email protected]
欧利希(湖北)医药科技有限公司17316404525 17316404525 · [email protected]
广东云星生物技术有限公司0751-18902340975/189023400307 18902340307 · [email protected]
伊势久(江苏连云港)生物科技有限责任公司18082049515 · [email protected]
武汉丰泰威远科技有限公司+86-27-8746-5837 +86-18627785180 · [email protected]
武汉丰泰威远科技有限公司027-87465837 19945049750 · [email protected]
常州协丰医药研发有限公司519-85601385 · [email protected]
天津希恩思生化科技有限公司400 638 7771 · [email protected]
斯百全化学(上海)有限公司021-021-021-67601398-809-809-809 15221380277 · [email protected]
深圳振强药物研发有限公司0755-66853366 13670046396 · [email protected]
南京杜莱生物技术有限公司025-84699383-8003;025-846993838003-8003 18013301590 · [email protected]
陕西麟邦实业有限公司17709134430 · [email protected]
天成化工(江苏)有限公司0512-66387919 18662433356 · [email protected]
深圳菲斯生物科技有限公司0755-83725350 13380397412 · [email protected]
上海将来实业股份有限公司021-61552785 · [email protected]
成都零六生物科技有限公司400-1166-196 13458535857 · [email protected]
天津普西唐生物医药科技有限公司010-60605840 · [email protected]
上海麦克林生化科技股份有限公司15221275939 15221275939 · [email protected]
武汉壹加壹生物科技有限公司027-027-16602739303 16602739303 · [email protected]
陕西缔都医药化工有限公司029-81024372 17792795018 · [email protected]
盖米科技(青岛)有限公司19524370118 · [email protected]
桦特昇生物科技(武汉)有限公司027-59207879 1972026995 18140587686 · [email protected]
九鼎化学(上海)科技有限公司4009209199 · [email protected]
资川生化科技(苏州)有限公司0512-66317708 13372112871 · [email protected]
上海谱振生物科技有限公司4001650900 13817534909 · [email protected]
广东翁江化学试剂有限公司0751-2886766 13927870850 · [email protected]
上海超岚化工科技中心(原宁波市超明生物科技有限公司)13301662590 · [email protected]
上海玻尔化学试剂有限公司19102157732 · [email protected]
武汉多普乐生物医药有限公司+86-02787215551 19945035818 · [email protected]
启源(广东)医药化工有限公司18026564465 · [email protected]
北京华威锐科化工科技有限公司89508211 18501085097 · [email protected]
艾万拓威达优尔国际贸易(上海)有限公司400-821-8006 · [email protected]

Additional notes

DefinitionChEBI: 3-amino-7-(dimethylamino)phenothiazin-5-ium is an organic cation that is phenothiazin-5-ium substituted by amino and dimethylamino groups at positions 3 and 7 respectively. The chloride salt is the histological dye 'azure A'.