N'-[N'-(2-phenylethyl)carbamimidoyl]ethanimidamide
EC 4.2.2.4 · units/mg solid · CHONDROITINASE ABC · chondroitinase ABC I · CHONDROITIN ABC LYASE · Lyase, chondroitin ABC · Endolyase, chondroitiBC · chondroitinase-abc bsa free
![N'-[N'-(2-phenylethyl)carbamimidoyl]ethanimidamide molecular structure](/img/chemical/168009893.png)
Identifiers & Physical Properties
| CAS No. | 9024-13-9 |
|---|---|
| Molecular formula | C99H144N36 |
| Average molar mass | 1,838.4 g/mol |
| Monoisotopic mass | 1,837.23746875 g/mol |
| SMILES | CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1.CC(N)=NC(N)=NCCc1ccccc1 |
| InChIKey | QSCUSRCQBGTCDB-UHFFFAOYSA-N |
| PubChem CID | 168009893 |
Chemical & Physical Properties
| Topological polar surface area | 691 |
|---|---|
| Hydrogen bond donors | 18 |
| Hydrogen bond acceptors | 9 |
| Rotatable bonds | 36 |
| Heavy atoms | 135 |
| Formal charge | 0 |
| Complexity | 239 |
| Storage conditions | -20°C |
| Solubility | 0.01% bovine serum albumin aqueous (BSA) solution: soluble |
| Physical state | lyophilized powder |
| Color | White to off-white |
| Biological source | bacterial (Proteus vulgaris) |
| Specific Activity | 50-250units/mg protein |
| Major applications | diagnostic assay manufacturing |
| FDA UNII | 7SI2UZG934 |
| UNSPSC Code | 12352204 |
| NACRES | NA.77 |
| Personal protective equipment (PPE) | Eyeshields, Gloves, type N95 (US) |
| Safety statements (S) | 22-24/25 |
| Water hazard class (WGK) | 3 |
| F | 10-21 |
| Storage class | 11 - Combustible Solids |
Safety & Regulations
| F | 10-21 |
|---|---|
| Personal protective equipment (PPE) | Eyeshields, Gloves, type N95 (US) |
| Water hazard class (WGK) | 3 |
| Safety statements (S) | 22-24/25 |
| Storage class | 11 - Combustible Solids |
Applications & Uses
Uses
Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan.
Uses
The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae.