HuagongsheHuagongshe Chemistry Print Report · For research reference only
Dehydrogenase, Formate
FDH · FORMATE DEHYDROGENASE · formate dehydrogenase from candida boidinii · IFDH · ADHX · CL274 · FALDH · GSNOR
Identifiers & Physical Properties
| CAS No. | 9028-85-7 |
|---|---|
| Molecular formula | C194H219BrO47S4 |
| Average molar mass | 3,511 g/mol |
| Monoisotopic mass | 3,507.28129 g/mol |
| SMILES | CCCc1ccc2cc(C=C(C)C(=O)c3c(O)cc(C(C)CCC=CCC(=O)OC)oc3=O)oc2c1.CCCc1ccc2cc(C=C(C)C(=O)c3c(O)cc(C(C)CCC=CCC(=O)OC)oc3=O)sc2c1.CCOc1ccc(C=C(C)C(=O)c2c(O)cc(C(C)CCC=CCC(=O)OC)oc2=O)cc1.CCOc1ccc(C=C(C)C(=O)c2c(O)cc(C(C)CCC=CCC(=O)OC)oc2=O)s1.COC(=O)CC=CCCC(C)c1cc(O)c(C(=O)C(C)=Cc2cc(Br)c(CC(C)C)s2)c(=O)o1.COC(=O)CC=CCCC(C)c1cc(O)c(C(=O)C(C)=Cc2cc3ccc(CCC(C)C)cc3o2)c(=O)o1.COC(=O)CC=CCCC(C)c1cc(O)c(C(=O)C(C)=Cc2ccc(Oc3ccc(C)cc3)s2)c(=O)o1 |
| InChIKey | MECJGSAIRDLPQG-UHFFFAOYSA-N |
| PubChem CID | 168009894 |
| EC No. | 232-844-2 |
Chemical & Physical Properties
| Topological polar surface area | 796 |
|---|---|
| Hydrogen bond donors | 7 |
| Hydrogen bond acceptors | 51 |
| Rotatable bonds | 85 |
| Heavy atoms | 246 |
| Formal charge | 0 |
| Complexity | 6,460 |
| Density | 1.1 g/mL at 20 °C |
| Storage conditions | 2-8°C |
| Physical state | powder |
| Color | clear brown |
| Biological source | yeast (Candida boidini) |
| Solubility | water: soluble 10g/L |
| Specific Activity | 5.0-15.0units/mg protein |
| EPA Substance Registry System | Dehydrogenase, formate (9028-85-7) |
| UNSPSC Code | 12352204 |
| NACRES | NA.54 |
| GHS pictogram | GHS08 |
| Signal word | Danger |
| GHS hazard statements | H334 |
| GHS precautionary statements | P261-P284-P501 |
| Personal protective equipment (PPE) | Eyeshields, Gloves, type N95 (US) |
| Water hazard class (WGK) | 3 |
| F | 10-21 |
| TSCA inventory status | TSCA listed |
| Storage class | 11 - Combustible Solids |
| Hazard class | Resp. Sens. 1 |
Safety & Regulations
| Regulatory information | XU - indicates a substance exempt from reporting under the Chemical Data Reporting Rule, (40 CFR 711).Dehydrogenase, formate: Does not have an individual approval but may be used under an appropriate group standard |
|---|---|
| F | 10-21 |
| Personal protective equipment (PPE) | Eyeshields, Gloves, type N95 (US) |
| Water hazard class (WGK) | 3 |
| TSCA inventory status | TSCA listed |
| Signal word | Danger |
| GHS pictogram | GHS08 |
| Storage class | 11 - Combustible Solids |
| GHS hazard statements | H334 |
| Hazard class | Resp. Sens. 1 |
| GHS precautionary statements | P261-P284-P501 |
Applications & Uses
Uses
Most widely used in cofactor recycling systems for NADH.
Uses
Formate Dehydrogenase (FDH) is used for diagnostics in large scale industrial processes. Its used in the production of an unnatural amino acid, tert-L-leucine, a component of some HIV protease and matrix metalloprotease inhibitors.
General Description
Formate:NAD+ oxidoreductaseFormate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.