1,4-Piperazinediethanesulfonic acid, potassium salt (1:2)
PIPES DIPOTASSIUM · PIPES 2K · PIPES-K2 · PIPES DIPOTASSIUM SALT · METHYLCELLULOSE 4000 CP · PIPES DIPOTASSIUM SALT, VETEC · PIPES dipotassiuM salt (PIPES-K{2}) · PIPES dipotassium salt, 99%, for bio
Identifiers & Physical Properties
| CAS No. | 108321-27-3 |
|---|---|
| Molecular formula | C8H16K2N2O6S2 |
| Average molar mass | 378.6 g/mol |
| Monoisotopic mass | 377.97239159 g/mol |
| SMILES | O=S(=O)([O-])CCN1CCN(CCS(=O)(=O)[O-])CC1.[K+].[K+] |
| InChIKey | BJQYFCAMUXGZFN-UHFFFAOYSA-L |
| PubChem CID | 176078 |
| DTXSID | DTXSID9073042 |
Chemical & Physical Properties
| Topological polar surface area | 138 |
|---|---|
| Hydrogen bond donors | 0 |
| Hydrogen bond acceptors | 8 |
| Rotatable bonds | 4 |
| Heavy atoms | 20 |
| Formal charge | 0 |
| Complexity | 370 |
| Chemical classes | Other Uses -> Biological Buffers |
| Physical Description | White solid[Sigma-Aldrich MSDS] |
| Storage conditions | Store at RT. |
| Solubility | H2O: 0.5 g/mL, clear, colorlesswater: 0.5g/mL, clear, colorless |
| Physical state | crystalline powder |
| pH | 6.1-7.5 |
| pKa (acidity coefficient) | 6.8(at 25℃) |
| PH Range | 6.1 - 7.5 |
| Major applications | diagnostic assay manufacturing |
| InChI | 1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)/q2*+1/p-2 |
| InChIKey | BJQYFCAMUXGZFN-UHFFFAOYSA-L |
| SMILES | [K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O |
| CAS DataBase Reference | 108321-27-3(CAS DataBase Reference) |
| EPA Substance Registry System | 1,4-Piperazinediethanesulfonic acid, dipotassium salt (108321-27-3) |
| UNSPSC Code | 12161700 |
| NACRES | NA.25 |
| GHS pictogram | GHS07 |
| Signal word | Warning |
| GHS hazard statements | H315-H319-H335 |
| GHS precautionary statements | P280-P302+P352-P362+P364 |
| Personal protective equipment (PPE) | Eyeshields, Gloves, type N95 (US) |
| Hazard symbols | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-37/39 |
| Water hazard class (WGK) | 3 |
| F | 10 |
| TSCA inventory status | TSCA listed |
| Customs code | 29335995 |
| Storage class | 11 - Combustible Solids |
Safety & Regulations
| Regulatory information | PMN - indicates a commenced PMN (Pre-Manufacture Notices) substance. |
|---|---|
| Hazards summary | Not considered hazardous by GHS classification[Sigma-Aldrich MSDS] See PIPES. |
| F | 10 |
| Personal protective equipment (PPE) | Eyeshields, Gloves, type N95 (US) |
| Water hazard class (WGK) | 3 |
| TSCA inventory status | TSCA listed |
| Customs code | 29335995 |
| Risk statements (R) | 36/37/38 |
| Signal word | Warning |
| GHS pictogram | GHS07 |
| Hazard symbols | Xi |
| Safety statements (S) | 26-37/39 |
| Storage class | 11 - Combustible Solids |
| GHS hazard statements | H315-H319-H335 |
| GHS precautionary statements | P280-P302+P352-P362+P364 |
Applications & Uses
Uses
PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.
General Description
PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.