Cfda-AM
Basic information
- CAS
124412-00-6- Standard SMILES
CC(=O)OCOC(=O)c1ccc2c(c1)C(=O)OC21c2ccc(OC(C)=O)cc2Oc2cc(OC(C)=O)ccc21- InChIKey
YDBUJZYYYBVSEF-UHFFFAOYSA-N- Molecular formula
- C28H20O11
- Average molar mass
- 532.4 g/mol
- Monoisotopic mass
- 532.10056145
Overview
is an indirect measure of cell membrane integrity-are noninvasive and can be monitored conveniently directly in multiwell plates
Names & identifiers
- Preferred name
- Cfda-AM
- IUPAC
- acetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
- English name
- 5-Cfda, am
- Molecular formula
- C28H20O11
- Molecular weight
- 532.45
Names & synonyms
124412-00-6CFDA-AM5-Carboxyfluorescein diacetate acetoxymethyl esterDTXSID90154324RefChem:302151DTXCID20768155-CFDA-AMacetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate5-carboxyfluorescein diacetate, acetoxymethyl ester5-CFDA, AMorb1941478SCHEMBL29672382
Show more names
5-Fam(Ac)2-AMYDBUJZYYYBVSEF-UHFFFAOYSA-NCFDAAMCarboxyfluoresceindiacetateAMester5-Carboxyfluorescein diacetate acetoxylmethyl ester5-Carboxyfluorescein diacetate (acetyloxy)methyl ester5-(AND-6)-CARBOXY SNARF(R)-1, ACETOXYMETHYL ESTER, ACETATE
5-CFDA-AM, 90%, single isomerDB-081114HY-131131CS-0129181T83293(Acetyloxy)methyl 3',6'-bis(acetyloxy)-3-oxo-3H-spiro[[2]benzofuran-1,9'-xanthene]-5-carboxylate(Acetyloxy)methyl 3',6'-bis(acetyloxy)-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylateSpiro(isobenzofuran-1(3H),9'-(9H)xanthene)-5-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-, (acetyloxy)methyl ester
Properties
Computed structural properties
- XLogP
- 3.5
- Topological polar surface area
- 141 Ų
- Hydrogen bond donors
- 0
- Hydrogen bond acceptors
- 11
- Rotatable bonds
- 9
- Heavy atoms
- 39
- Formal charge
- 0
- Complexity
- 967
Physicochemical properties
- Boiling point
- 737 · 737.0±60.0 °C(Predicted)
- Density
- 1.51 · 1.51±0.1 g/cm3(Predicted)
- form
- Solid
- color
- White to off-white
- Appearance
- Solid Powder
Chemistry & industry
Applications
Description5-carboxyfluorescein diacetate acetoxymethyl ester is a cell-permeant fluorogenic esterase substrate. It can be converted by nonspecific esterases of living cells from a membrane-permeable, nonpolar, nonfluorescent substance to a polar product CF, indicating the integrity of the plasma membrane.
Uses5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].
Related reactions
0 records
No reaction records under the current filter.
Compound notes
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