Basic information
- CAS
4196-99-0- Standard SMILES
O=S(=O)([O-])c1ccc(N=Nc2ccc(N=Nc3c(O)ccc4ccccc34)c(S(=O)(=O)[O-])c2)cc1.[Na+].[Na+]- InChIKey
VVAVKBBTPWYADW-UHFFFAOYSA-L- Molecular formula
- C22H14N4Na2O7S2
- Average molar mass
- 556.5 g/mol
- Monoisotopic mass
- 556.00992971
Overview
Biebrich scarlet is an organic sodium salt having 2-[(2-hydroxynaphthalen-1-yl)diazenyl]-5-[(4-sulfonatophenyl)diazenyl]benzene-1-sulfonate as the counterion. Used as a plasma stain in Masson's trichrome. It has a role as a histological dye. It contains a Biebrich scarlet(2-).
Names & identifiers
- Preferred name
- Sodium 6-(2-hydroxynaphthylazo)-3,4'-azodibenzenesulphonate
- IUPAC
- disodium;2-[(2-hydroxynaphthalen-1-yl)diazenyl]-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate
- English name
- Acid red 66
- Molecular formula
- C22H17N4NaO7S2
- Molecular weight
- 536.51
Names & synonyms
Biebrich scarletPonceau BSCroceine scarletAcid Scarlet BAFast Scarlet BAFenazo Scarlet BScarlet 3BC.I. Acid Red 66Imperial Scarlet 3BImperial Scarlet 3BNCalcochrome Red 650Naphthazine Scarlet BI
Show more names
CI 26905NSC79710SCARLET BPONCEAU BNSC 79710NSC-79710BONCEAU BSScarlet ECCI NO 26905
Amacid Chrome Red 3BC.I. 26905Scarlet Red Sulfonate NFSodium 6-(2-hydroxynaphthylazo)-3,4'-azodibenzenesulphonateC.I. ACID RED 66, DISODIUM SALTDTXSID2063345CHEBI:87186RefChem:119763
Database identifiers
- CAS
4196-99-0- PubChem CID
- 20170
- DTXSID
DTXSID2063345- ChEBI
CHEBI:87186- EC Number
- 224-084-5
Properties
Computed structural properties
- Topological polar surface area
- 201 Ų
- Hydrogen bond donors
- 1
- Hydrogen bond acceptors
- 11
- Rotatable bonds
- 4
- Heavy atoms
- 37
- Formal charge
- 0
- Complexity
- 958
Physicochemical properties
- Melting point
- 181 · 181-188 °C
- Flash point
- 300 ° · 300°(572°F)
- storage temp.
- room temp
- solubility
- 1 mg/mL · ethanol: 1 mg/mL
- form
- Powder
- Colour Index
- 26905 · 26905
- color
- Dark brown powder
- Water Solubility
- Appreciable (> 10%)
- λmax
- 505 nm · 505 nm
- BRN
- 3883900 · 3883900
- Stability
- Stable. Incompatible with strong oxidizing agents.
- Major Application
- diagnostic assay manufacturinghematologyhistology
- InChIKey
- VVAVKBBTPWYADW-RVTJCSDESA-L
- SMILES
- [Na+].[Na+].Oc1ccc2ccccc2c1\N=N\c3ccc(cc3S([O-])(=O)=O)\N=N\c4ccc(cc4)S([O-])(=O)=O
- CAS DataBase Reference
- 4196 · 4196-99-0
- EPA Substance Registry System
- 66 · C.I. Acid Red 66, disodium salt (4196-99-0)
- UNSPSC Code
- 12171500 · 12171500
- NACRES
- 47 · NA.47
- Symbol(GHS)
- 7 · GHS07
- Signal word
- Warning
- Hazard statements
- 315 · H315-H319-H335
- Precautionary statements
- 264 · P264-P280-P305+P351+P338-P337+P313P-P264-P280-P302+P352-P321-P332+P313-P362
- PPE
- 95 · Eyeshields, Gloves, type N95 (US)
- Hazard Codes
- Xi,Xn
- Risk Statements
- 36 · 36/37/38-20/21/22
- Safety Statements
- 37 · 37/39-26-36
- WGK Germany
- 3 · 3
- TSCA
- TSCA listed
- HS Code
- 32041200 · 32041200
- Storage Class
- 11 · 11 - Combustible Solids
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
GHS classification
GHS Classification
This chemical does not meet GHS hazard criteria for 66.7% (6 of 9) of all reports.
Warning
H302 (11.1%): Harmful if swallowed [Warning Acute toxicity, oral]
P261, P264, P264+P265, P270, P271, P280, P301+P317, P302+P352, P304+P340, P305+P351+P338, P317, P319, P321, P330, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)
Aggregated GHS information provided per 9 reports by companies from 4 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.
Hazard information
Regulatory Information
Chemical: Benzenesulfonic acid, 2-[(2-hydroxy-1-naphthalenyl)azo]-5-[(4-sulfophenyl)azo]-, disodium salt
Benzenesulfonic acid, 2-(2-hydroxy-1-naphthalenyl)azo-5-(4-sulfophenyl)azo-, disodium salt: Does not have an individual approval but may be used under an appropriate group standard
Other Safety InformationIMAP assessments - Benzenesulfonic acid, 2-[(2-hydroxy-1-naphthalenyl)azo]-5-[(4-sulfophenyl)azo]-, disodium salt: Human health tier I assessment
Hazard Classes and CategoriesAcute Tox. 4 (11.1%)
Safety data
- PPE
- Eyeshields, Gloves, type N95 (US)
- wgk
- 3
- tsca
- TSCA listed
- hs_code
- 32041200
- risk_code
- 36/37/38-20/21/22
- Signal word
- Warning
- Symbol(GHS)
- GHS07
- hazard_code
- Xi,Xn
- safety_code
- 37/39-26-36
- Storage Class
- 11 - Combustible Solids
- Hazard statements
- H315-H319-H335
- Precautionary statements
- P264-P280-P305+P351+P338-P337+P313P-P264-P280-P302+P352-P321-P332+P313-P362
Chemistry & industry
Uses & manufacturing
General Manufacturing InformationBenzenesulfonic acid, 2-[2-(2-hydroxy-1-naphthalenyl)diazenyl]-5-[2-(4-sulfophenyl)diazenyl]-, sodium salt (1:2): ACTIVE
Applications
UsesPonceau BS has been used in Masson′s trichrome stain for the staining of collagen. It has also been used for eosinophil staining.
Preparation methods
Preparation3,4′-Disulfo-4-aininoazobenzenediazo, and Naphthalen-2-ol coupling.
Upstream & downstream products
Upstream raw materials
Suppliers81 total
国药集团化学试剂有限公司86-21-63210123 · [email protected]
北京华美互利生物化工010-56205725 · [email protected]
上海阿拉丁生化科技股份有限公司400-6206333 13167063860 · [email protected]
湖南韵邦生物科技股份有限公司0731-88532326 13319508186 · [email protected]
湖北威德利化学试剂有限公司186-02735115 18062128043 · [email protected]
上海康朗生物科技有限公司021-61998208 18217752821 · [email protected]
上海麦克林生化科技股份有限公司021-50706066 15221275939 · [email protected]
上海哲研生物科技有限公司021-18017610038 18017610038 · [email protected]
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金克隆（北京）生物技术有限公司400-910-1997 15330005590 · [email protected]
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上海谱科生物技术有限公司021-64705760 18027310741 · [email protected]
Sigma-Aldrich西格玛奥德里奇(上海)贸易有限公司021-61415566 800-8193336 · [email protected]
天津荣燊化工有限公司18317763565; 18317763565 · [email protected]
桦特昇生物科技（武汉）有限公司027-59207879 1972026995 18140587686 · [email protected]
上海陶术生物科技股份有限公司+1-781-999-5354; +1-00000000000 · [email protected]
北京诺其雅盛生物科技有限公司4000-819-385 010-62329685 13718666987
九鼎化学（上海）科技有限公司4009209199 · [email protected]
Henan Alfa Chemical Co., Ltd+8618339805032 · [email protected]
上海谱振生物科技有限公司4001650900 13817534909 · [email protected]
广东翁江化学试剂有限公司0751-2886766 13927870850 · [email protected]
上海玻尔化学试剂有限公司19102157732 · [email protected]
浙江珲达生物科技有限公司0571-89903022 18679456698 · [email protected]
武汉福鑫化工有限公司+86-2759207851; +86-18007136271 · [email protected]
艾万拓威达优尔国际贸易(上海)有限公司400-821-8006 · [email protected]
上海西陇生化科技有限公司021-52907766-8042
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山东小野化学股份有限公司0539-6362799 20）
Additional notes
DefinitionChEBI: An organic sodium salt having 2-[(2-hydroxynaphthalen-1-yl)diazenyl]-5-[(4-sulfonatophenyl)diazenyl]benzene-1-sulfonate as the counterion. Used as a plasma stain in Masson's trichrome.
Related reactions
0 records
No reaction records under the current filter.
Compound notes
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