C.I. Acid Green 3

LC50; Species: Oryzias latipes (Medaka) length 2 cm, weight 0.2 g; Conditions: freshwater, static, 25 °C; Concentration: 1000000 ug/L for 24 hr /formulation/

LC50; Species: Oryzias latipes (Medaka) length 2 cm, weight 0.2 g; Conditions: freshwater, static, 25 °C; Concentration: 990000 ug/L for 48 hr /formulation/

TERRESTRIAL FATE: Acid dyes, such as C.I. Acid Green 3, typically exhibit very low solubilities in octanol and have water solubilities that exceed 100 g/L. Therefore, these compounds would be expected to remain in the water column in the aquatic environment and show little affinity for organic matter(1) which suggests a potential to leach in soil(SRC). Volatilization of C.I. Acid Green 3 from moist soil surfaces is not expected to be an important fate process(SRC) given an estimated Henry's Law constant of 4.9X10-29 atm-cu m/mole(SRC), using a fragment constant estimation method(2). C.I. Acid Green 3 not expected to volatilize from dry soil surfaces(SRC) based upon an estimated vapor pressure of 7.2X10-39 mm Hg at 25 °C(SRC), determined from a fragment constant method(2). Biodegradation data in soil were not available(SRC, 2011).

AQUATIC FATE: Acid dyes, such as C.I. Acid Green 3, typically exhibit very low solubilities in octanol and have water solubilities that exceed 100 g/L. Therefore, these compounds would be expected to remain in the water column in the aquatic environment and show little affinity for organic matter(1). Volatilization from water surfaces is not expected(2) based upon an estimated Henry's Law constant of 4.9X10-29 atm-cu m/mole(SRC), developed using a fragment constant estimation method(3). According to a classification scheme(4), an estimated BCF of 3(SRC), from an estimated log Kow of -3.20(3) and a regression-derived equation(3), suggests the potential for bioconcentration in aquatic organisms is low(SRC). Biodegradation data in water were not available(SRC, 2011).

ATMOSPHERIC FATE: According to a model of gas/particle partitioning of semivolatile organic compounds in the atmosphere(1), C.I. Acid Green 3, which has an estimated vapor pressure of 7.2X10-39 mm Hg at 25 °C(SRC), determined from a fragment constant method(2), is expected to exist solely in the particulate phase in the ambient atmosphere. Particulate-phase C.I. Acid Green 3 may be removed from the air by wet or dry deposition(SRC). C.I. Acid Green 3 absorbs light at wavelengths of 618 nm(4), and therefore may be susceptible to direct photolysis by sunlight(SRC).

Acid dyes, such as C.I. Acid Green 3, typically exhibit very low solubilities in octanol and have water solubilities that exceed 100 g/L. Therefore, these compounds would be expected to remain in the water column in the aquatic environment and show little affinity for organic matter(1).

/OTHER TOXICITY INFORMATION/ Most organic azo dyes are potential skin sensitizers, the most important of which are paraphenylenediamine and its analogs. Water soluble azo dyes are more likely to cause clinical sensitization than insoluble dyes. ... In addition to allergic eczematous contact dermatitis, color developing solutions have caused lichen planus like eruptions. /Organic dyes/

Guinea Green B

Group 3: Not classifiable as to its carcinogenicity to humans

Volume 16: (1978) Some Aromatic Amines and Related Nitro Compounds – Hair Dyes, Colouring Agents and Miscellaneous Industrial Chemicals

1987

1987

An estimated BCF of 3 was calculated in fish for C.I. Acid Green 3(SRC), using an estimated log Kow of -3.20(1) and a regression-derived equation(1). According to a classification scheme(2), this BCF suggests the potential for bioconcentration in aquatic organisms is low(SRC).

The Henry's Law constant for C.I. Acid Green 3 is estimated as 4.9X10-29 atm-cu m/mole(SRC) using a fragment constant estimation method(1). This Henry's Law constant indicates that C.I. Acid Green 3 is expected to be essentially nonvolatile from water surfaces(2). C.I. Acid Green 3's Henry's Law constant indicates that volatilization from moist soil surfaces is not expected occur(SRC). C.I. Acid Green 3 is not expected to volatilize from dry soil surfaces(SRC) based upon an estimated vapor pressure of 7.2X10-39 mm Hg(SRC), determined from a fragment constant method(1).

/LABORATORY ANIMALS: Acute Exposure/ Acid green was not damaging in rabbit eye test. It caused no more than slight transient irritation when tested on the eyes of rabbits, either by application of 4-8 mg of powdered dye to the conjunctival sac or by irrigating the surface of the eye for ten minutes with 0.02 M solution near neutrality after the epithelium has been removed to facilitate penetration. ...

/LABORATORY ANIMALS: Acute Exposure/ Arylmethane dyes which have been utilized as food colors, commercial dyes, laboratory stains and pH indicators were tested in the Salmonella/mammalian microsome mutagenicity assay. Eight mutagenic dyes were identified, including 5 food colors and 3 common laboratory stains; none of the 11 indicator dyes tested were mutagenic. The food color ... guinea green C.I. 42085 ... /was/ mutagenic in frameshift mutation detector strains TA98 and/or TA1538 and required metabolic activation. Most of the compounds gave weak mutagenic responses with Salmonella and were positive only within narrow dose ranges. Since conflicting results were obtained using dyes from different sources, minor dye components may have been responsible for their mutagenicity. Impurities in arylmethane colors still used in food and the toxicological role of such impurities merits further study.

/LABORATORY ANIMALS: Chronic Exposure or Carcinogenicity/ Toxicological testing of food dyes was conducted with experimental rats. ... Green-No-2 fed to rats at 2 percent of the diet for two years caused no tumors. With the remaining four dyes, Green-No-1 (Guinea-Green-B), Red-No-2 (amaranth), Red-No-3 (erythrosine), and Yellow-No-5 (tartrazine), no distant tumors were caused by the injections; except for one with Green-No-1, none appeared at the point of injection. Some of the animals survived, and feeding studies on some of the dyes were continued.

/LABORATORY ANIMALS: Chronic Exposure or Carcinogenicity/ The results of feeding rats, dogs and mice diets containing Guinea Green B, Light Green SF Yellowish or Fast Green FCF for 2 yr are reported. ... The three green colorings, Guinea Green B, Light Green SF Yellowish and Fast Green FCF, were each fed at dietary levels of 0.0, 1.0 and 2.0 % to groups of two male and two female beagle dogs for 2 yr. Ten of the 12 dogs fed Guinea Green B survived in good clinical condition. A male at 2 % was killed after 1 yr when he became moribund and a female at 1% died after 5 months. Both non-survivors showed generalized inanition-type atrophy of the bone marrow, fat, skeletal muscle, adrenal cortex, lymphatic tissue and the gastric mucosa. Moderate periportal fatty metamorphosis of the liver was observed in a male survivor on 2 %. ...

For more Non-Human Toxicity Excerpts (Complete) data for C.I. ACID GREEN 3 (11 total), please visit the HSDB record page.