2-[4-(2-Hydroxyethyl)piperazin-4-ium-1-yl]ethanesulfonate

DescriptionHEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

UsesNon-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

UsesHEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.HEPES has been used as a component of:Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.

UsesHEPES is a biological buffer. It may be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for use with the Folin-Ciocalteu protein assay.

General DescriptionHEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.