Direct Blue 218

EYES: First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center. Do not put any ointments, oils, or medication in the victim's eyes without specific instructions from a physician. IMMEDIATELY transport the victim after flushing eyes to a hospital even if no symptoms (such as redness or irritation) develop.

SMALL SPILLS AND LEAKAGE: If you spill this chemical, you should dampen the solid spill material with water, then transfer the dampened material to a suitable container. Use absorbent paper dampened with water to pick up any remaining material. Seal your contaminated clothing and the absorbent paper in a vapor-tight plastic bag for eventual disposal. Wash all contaminated surfaces with a soap and water solution. Do not reenter the contaminated area until the Safety Officer (or other responsible person) has verified that the area has been properly cleaned.

SRP: At the time of review, criteria for land treatment or burial (sanitary landfill) disposal practices are subject to significant revision. Prior to implementing land disposal of waste residue (including waste sludge), consult with environmental regulatory agencies for guidance on acceptable disposal practices.

RECOMMENDED RESPIRATOR: Where the neat test chemical is weighed and diluted, wear a NIOSH-approved half face respirator equipped with an organic vapor/acid gas cartridge (specific for organic vapors, HCl, acid gas and SO2) with a dust/mist filter. (NTP, 1992)

The following link will take the user to the National Toxicology Program (NTP) Test Agent Search Results page, which tabulates all of the "Standard Toxicology & Carcinogenesis Studies", "Developmental Studies", and "Genetic Toxicity Studies" performed with this chemical. Clicking on the "Testing Status" link will take the user to the status (i.e., in review, in progress, in preparation, on test, completed, etc.) and results of all the studies that the NTP has done on this chemical. [http://ntp-apps.niehs.nih.gov/ntp_tox/index.cfm?fuseaction=ntpsearch.searchresults&searchterm=28407-37-6]

C.I. direct blue 218 (CAS # 28407-37-6) was evaluated for acute dermal toxicity. The test substance was applied to the clipped backs of New Zealand white rabbits (1/sex abraded; 1/sex intact) at a dose level of 2.0 g/kg of body weight. The test site was covered for 24-hours. Diarrhea, bloated abdomen and few feces was noted in animal #1; and lethargy in animal #2. All other animals appeared normal. Necropsy findings concluded that all animals appeared normal. No mortality occurred and the dermal LD50 was determined to be greater than 2.0 g/kg b.w.

C.I. direct blue 218 (CAS # 28407-37-6) was evaluated for acute inhalation toxicity. The test substance was administered by a direct dust-feed system to Holtzman Sprague-Dawley rats (5/sex) at a concentration of 11.71 mg/l for 4-hours. No mortality occurred. No clinical signs occurred during exposure or the 14-day observation period. Body weights of males were normal, but 2 females exhibited slight depressed weight gain. Necropsy findings found macroscopic lesions in the lungs of all animals. These findings included hemorrhagic spots and foci, a purplish discoloration and rough texture of lobes or portions of lobes, and nodular masses or growths with a shrunken appearance. These findings were not considered treatment-related because it was concluded that these findings were typical of chronic murine pneumonia complex.

C.I. direct blue 218 (CAS # 28407-37-6) was evaluated for eye irritation. The test substance was instilled in the lower conjunctival sac of one eye of each of 6 New Zealand white rabbits at a dosage of 0.1 ml (liquid) or 0.1 g (solid). The lids were held closed for 1-second for even distribution of the test substance. The test substance was determined to be moderately irritating. No further information was submitted.

C.I. direct blue 218 (CAS # 28407-37-6) was evaluated for primary dermal irritation. The test substance was applied to the clipped back and sides (one-side was abraded) to 6 New Zealand white rabbits at a dosage of 0.5 ml (liquid) or 0.5 g (solid) and covered for 24-hours. The test substance was determined to be non-irritating. No further information was submitted.

For more TSCA Test Submissions (Complete) data for DIRECT BLUE 218 (12 total), please visit the HSDB record page.

CI Direct Blue 218

Group 2B: Possibly carcinogenic to humans

Volume 129: (2022) Gentian Violet, Leucogentian Violet, Malachite Green, Leucomalachite Green, and CI Direct Blue 218

2022 online

2021

C.I. Direct Blue 218

Direct Blue 218 was evaluated for the induction of sex-linked recessive lethal mutations in Drosophila melanogaster by the National Toxicology Program. Canton-S wild-type males were treated with concentrations of Direct Blue 218 that result in approximately 30% mortality. Following treatment, males were mated individually to 3 harems of Basc virgin females to produce 3 broods for analysis. The concentrations of Direct Blue 218 tested by injection (1000 ppm) or feeding (10,000 ppm) were negative in this assay.

Under the conditions of these 2 yr feed studies, there was some evidence of carcinogenic activity of C.I. Direct Blue 218 in male F344/N rats based on the occurrence of pharyngeal neoplasms. Squamous cell neoplasms of the forestomach may have been chemical related. There was no evidence of carcinogenic activity of C.I. Direct Blue 218 in female F344/N rats given 1,000, 3,000 or 10,000 ppm. There was clear evidence of carcinogenic activity of C.I. Direct Blue 218 in male and female B6C3F1 mice based upon incr incidences of hepatocellular adenomas and carcinomas. The occurrence of a few neoplasms of the kidney and small intestine in male mice may have been related to C.I. Direct Blue 218 treatment. ...

C.I Direct Blue 218 was not mutagenic in Salmonella typhimurium strains TA98, TA100, TA1535, or TA1537 tested with and without exogenous metabolic activation (S9). It was also tested in a modified Salmonella test protocol which employed reductive metabolism supplied by flavin mononucleotide or rat cecal bacteria, followed by oxidative metabolism; results of this test using strain TA1538 were also negative. C.I. Direct Blue 218 induced a small but significant increase in sister chromatid exchanges in Chinese hamster ovary cells at the highest dose tested without S9. No increase in chromosomal aberrations were observed in Chinese hamster ovary cells with or without S9. C.I. Direct Blue 218 did not induce sex linked recessive lethal mutations in germ cells of male Drosophila melanogaster.

... Toxicology and carcinogenesis studies were conducted by admin C.I. Direct Blue 218 in feed to groups of male and female F344/N rats and B6C3F1 mice for ... 2 yr. ... Groups of 60 male and 60 female rats were fed diets containing 0, 1,000, 3,000 or 10,000 ppm C.I. Direct Blue 281 for 103 wk. ... Groups of 60 male and 60 female mice were fed diets containing 0, 1,000, 3,000 or 10,000 ppm C.I. Direct Blue 218 for 103 wk. ... Under the conditions of these 2 yr feed studies, there was some evidence of carcinogenic activity of C.I. Direct Blue 218 in male F344/N rats based on the occurrence of pharyngeal neoplasms. Squamous cell neoplasms of the forestomach may have been chemical related. There was no evidence of carcinogenic activity of C.I. Direct Blue 218 in female F344/N rats given 1,000, 3,000 or 10,000 ppm. There was clear evidence of carcinogenic activity of C.I. Direct Blue 218 in male and female B6C3F1 mice based upon incr incidences of hepatocellular adenomas and carcinomas. The occurrence of a few neoplasms of the kidney and small intestine in male mice may have been related to C.I. Direct Blue 218 treatment. ...

0.14000000000000001

CALEPA

500

CALEPA