4-((4-(DIMETHYLAMINO)PHENYL)AZO)BENZOIC ACID, SUCCINIMIDYL ESTER

DescriptionDabcyl SE is an amine-reactive, chromophoric dye that is useful in FRET (fluorescence resonance energy transfer) applications. It is utilized in the synthesis of quenched nucleic acid probes and protease substrates. These substrates are non-fluorescent until an enzymatic event liberates the fluorophore from the quencher resulting in a fluorescent response.

UsesNon-Fluorescent probe for labeling proteins or peptides

Uses4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester is a useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies. It is also used for N-terminal modification of peptides in automated synthesis.