Basic information
- CAS
81-93-6- Standard SMILES
Nc1ccc2nc3ccc(N)cc3[n+](-c3ccccc3)c2c1.[Cl-]- InChIKey
SOUHUMACVWVDME-UHFFFAOYSA-N- Molecular formula
- C18H15ClN4
- Average molar mass
- 322.8 g/mol
- Monoisotopic mass
- 322.0985242
Overview
Phenosafranine is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. It has a role as a photosensitizing agent, a fluorochrome and a histological dye. It contains a 3,7-diamino-5-phenylphenazin-5-ium.
Names & identifiers
- Preferred name
- Phenosafranine
- IUPAC
- 10-phenylphenazin-10-ium-2,8-diamine chloride
- English name
- Phenosafranin
- Molecular formula
- C18H15ClN4
- Molecular weight
- 322.8
Names & synonyms
PhenosafraninPhenosafranine81-93-6Phenosafranine, chloridePhenosafrininePhenosofraninePhenosofrinine3,7-Diamino-5-phenylphenazinium chlorideSafranin B ExtraSafrinin B ExtraSchultz No. 958C.I. 50200
Show more names
nsc9855CI 50200CI NO 50200schultzno.958Phenosafeaninesafrininbextra
47U3T2FC2WNSC-9855NSC-215209RefChem:172892201-387-03,7-diamino-5-phenylphenazin-5-ium chloridePhenazinium, 3,7-diamino-5-phenyl-, chlorideUNII-47U3T2FC2W
Database identifiers
- CAS
81-93-6- PubChem CID
- 65732
- DTXSID
DTXSID701001694- ChEMBL
CHEMBL1968093、CHEMBL355307- ChEBI
CHEBI:33601- UNII
- 47U3T2FC2W
- EC Number
- 201-387-0
Properties
Computed structural properties
- Topological polar surface area
- 68.8 Ų
- Hydrogen bond donors
- 2
- Hydrogen bond acceptors
- 4
- Rotatable bonds
- 1
- Heavy atoms
- 23
- Formal charge
- 0
- Complexity
- 355
Experimental & physicochemical properties
Chemical ClassesDyes -> Azine Dyes
Physical DescriptionGreen lustrous solid; [Merck Index] Powder; [Sigma-Aldrich MSDS]
Physicochemical properties
- Melting point
- 300 °C · 300 °C
- Boiling point
- 478.49 °C · 478.49°C (rough estimate)
- Density
- 1.1738 · 1.1738 (rough estimate)
- refractive index
- 1.611 · 1.6110 (estimate)
- storage temp.
- room temp
- Colour Index
- 50200 · 50200
- form
- Solid
- color
- Green to dark green
- λmax
- 519 nm · 519 nm
- Merck
- 13 · 13,7333
- BRN
- 3642082 · 3642082
- Major Application
- diagnostic assay manufacturinghematologyhistology
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
GHS classification
GHS Classification
Warning
H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]
P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)
Aggregated GHS information provided per 40 reports by companies from 3 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.
Hazard information
Hazards SummaryIntravenous LD50 (mouse) = 18 mg/kg; [RTECS] A skin and strong eye irritant; [Sigma-Aldrich MSDS]
Hazard Classes and CategoriesSkin Irrit. 2 (100%)
Safety data
- PPE
- dust mask type N95 (US), Eyeshields, Gloves
- wgk
- 3
- rtecs
- SG1630000
- risk_code
- 36/37/38
- Signal word
- Warning
- Symbol(GHS)
- GHS07
- hazard_code
- Xi
- safety_code
- 26-36
- Storage Class
- 11 - Combustible Solids
- target organs
- Respiratory system
- Hazard statements
- H315-H319-H335
- Hazard Classifications
- Eye Irrit. 2Skin Irrit. 2STOT SE 3
Chemistry & industry
Uses & manufacturing
UsesUsed as a biological stain; [Merck Index]
Applications
UsesPhenosafranin is a bacterial stain used in microscopy, biological stain, and nuclear staining.
Suppliers77 total
国药集团化学试剂有限公司86-21-63210123 · [email protected]
上海阿拉丁生化科技股份有限公司400-6206333 13167063860 · [email protected]
湖南韵邦生物科技股份有限公司0731-88532326 13319508186 · [email protected]
上海康朗生物科技有限公司021-61998208 18217752821 · [email protected]
上海哲研生物科技有限公司021-18017610038 18017610038 · [email protected]
杭州品高化工有限公司0571-87658025 · [email protected]
上海毕得医药科技股份有限公司400-1647117 13681763483 · [email protected]
Suzhou ARTK Medchem Co., Ltd.+86-18168183658 · [email protected]
上海如吉生物科技发展有限公司+86-21-65211385-8001 36031160 · [email protected]
上海弘顺生物科技有限公司15901914505 · [email protected]
北京华威锐科化工有限公司
Related reactions
0 records
No reaction records under the current filter.
Compound notes
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