Basic information
- CAS
92-31-9- Standard SMILES
Cc1cc2nc3ccc(=[N+](C)C)cc-3sc2cc1N.[Cl-]- InChIKey
GEDVVYWLPUPJJZ-UHFFFAOYSA-N- Molecular formula
- C15H16ClN3S
- Average molar mass
- 305.8 g/mol
- Monoisotopic mass
- 305.0753464
Overview
Tolonium chloride is an organic chloride salt having 3-amino-7-(dimethylamino)-2-methylphenothiazin-5-ium (tolonium) as the counterion. It is a blue nuclear counterstain that can be used to demonstrate Nissl substance and is also useful for staining mast cell granules, both in metachromatic and orthochromatic techniques. It has a role as a diagnostic agent, a fluorochrome and a histological dye. It contains a tolonium.
Names & identifiers
- Preferred name
- Tolonium chloride
- IUPAC
- (7-amino-8-methylphenothiazin-3-ylidene)-dimethylazanium chloride
- English name
- Toluidine Blue O
- Molecular formula
- C15H16ClN3S
- Molecular weight
- 305.82
Names & synonyms
Toluidine Blue OTOLONIUM CHLORIDE92-31-9Basic Blue 17Blutene chlorideMenodinGabilinTolazulC.I. Basic Blue 17Toluidine Blue OOKlotToloni chloridum
Show more names
TBTOLUIDINE BLUEBASIC BLUEfklotCI 52040
F KlotBluteneSchultz 1041Cloruro de tolonioChlorure de toloniumToluidene Blue O ChlorideDimethyltoluthionine chlorideSchultz no. 1041
Database identifiers
- CAS
92-31-9- PubChem CID
- 7083
- DTXSID
DTXSID9048728- ChEMBL
CHEMBL1790006- ChEBI
CHEBI:87647- UNII
- 15XUH0X66N
Properties
Computed structural properties
- Topological polar surface area
- 66.7 Ų
- Hydrogen bond donors
- 1
- Hydrogen bond acceptors
- 4
- Rotatable bonds
- 0
- Heavy atoms
- 20
- Formal charge
- 0
- Complexity
- 511
Physicochemical properties
- Melting point
- 200 ℃ · >200℃
- bulk density
- 410 kg/m3 · 410kg/m3
- storage temp.
- Inert atmosphere,Room Temperature
- solubility
- 2 O: · H2O: soluble1mg/mL
- form
- Solid
- pka
- 2.4 · 2.4, 11.6(at 25℃)
- Colour Index
- 52040 · 52040
- color
- Dark green to black
- PH
- 2.8 · 2.8 (H2O, 25℃)
- Water Solubility
- 3.82 g/100 · 3.82 g/100 mL
- λmax
- 626 nm, · 626 nm, 630 nm
- ε(extinction coefficient)
- 13000 at · ≥13000 at 241-247nm in H2O≥28000 at 287-293nm in H2O≥40000 at 625-635nm in H2O
- Merck
- 14 · 14,9520
- Stability
- Stable. Incompatible with strong oxidizing agents.
- Biological Applications
- 45 · Bound hyaluronan assay; DNA assay; bioelectronic applications; biofuel cells; microbial fuel cells; detecting bacteria,body fluid,nucleic acids,45,proteins,viruses; identifying Gram-negative bacteria,b-amyloid reducing agents
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
Safety data
- wgk
- 3
- tsca
- TSCA listed
- rtecs
- SP5670000
- hs_code
- 32041300
- Toxicity
- LD50 in mice, rats, rabbits (mg/kg): 27.56, 28.93, 13.44 i.v. (Haley, Storlarsky)
- risk_code
- 36/37/38
- Signal word
- Warning
- Symbol(GHS)
- GHS07
- safety_code
- 22-24/25
- Storage Class
- 11 - Combustible Solids
- Hazard statements
- H302
- Precautionary statements
- P280-P305+P351+P338
Chemistry & industry
Applications
UsesToluidine Blue O is a metachromatic dye suitable for a wide variety of histological staining procedures. It is used for direct dyeing and printing of wool and silk, as well as a biological stain and hemostatic agent.
Preparation methods
Preparationby heavy chromate salt in the presence of sodium content and deal with N1,N1-dimethylbenzene-1,4-diamine, transforming it into the content and sulfonic acid, this product then through heavy chromate salt and O-Methylaniline processing, into a unique of sulfur acid amine generation, then use heavy chromate salt and copper sulfate closed loop and oxidation, Finally,thezinc chloridesaltprecipitation
Upstream & downstream products
Upstream raw materials
Suppliers100 total
国药集团化学试剂有限公司86-21-63210123 · [email protected]
北京华美互利生物化工010-56205725 · [email protected]
梯希爱(上海)化成工业发展有限公司
Related reactions
0 records
No reaction records under the current filter.
Compound notes
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