Basic information
- CAS
92-32-0- Standard SMILES
CN(C)c1ccc2cc3ccc(=[N+](C)C)cc-3oc2c1.[Cl-]- InChIKey
INCIMLINXXICKS-UHFFFAOYSA-M- Molecular formula
- C17H19N2O.Cl
- Average molar mass
- 302.8 g/mol
- Monoisotopic mass
- 302.1185909
Overview
Pyronin Y is an organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna-Pappenheim method. It has a role as a histological dye. It is an iminium salt and an organic chloride salt. It contains a pyronin Y cation.
Names & identifiers
- Preferred name
- Pyronine
- IUPAC
- [6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium chloride
- English name
- PYRONIN Y
- Molecular formula
- C17H19ClN2O
- Molecular weight
- 302.8
Names & synonyms
Pyronin Y92-32-0Pyronine YPyroninePyronin GPyronin YellowPyronine GTetramethyl pyronin3,6-Bis(dimethylamino)xanthylium chlorideMethyl pyroninXanthylium, 3,6-bis(dimethylamino)-, chloridePyronine ZH
Show more names
Pyonin GPyonin YCI 45005PYRONIN J
C.I. 45005tetramethyldiaminoxanthylium chlorideSchultz No. 853NSC-10454W659G165T1CHEBI:8682DTXSID8059051CI-45005
Database identifiers
- CAS
92-32-0- PubChem CID
- 7085
- DTXSID
DTXSID8059051- ChEMBL
CHEMBL1486690- ChEBI
CHEBI:8682- UNII
- W659G165T1
- EC Number
- 202-147-8
Properties
Computed structural properties
- Topological polar surface area
- 15.5 Ų
- Hydrogen bond donors
- 0
- Hydrogen bond acceptors
- 3
- Rotatable bonds
- 1
- Heavy atoms
- 21
- Formal charge
- 0
- Complexity
- 480
Experimental & physicochemical properties
Chemical ClassesDyes -> Acridine Dyes
Physical Description
Liquid
Lustrous green solid; [Merck Index] Iridescent green crystals; [Amresco MSDS]
Physicochemical properties
- Melting point
- 250 · 250-260 °C(lit.)
- storage temp.
- room temp
- solubility
- Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve
- form
- Powder/Solid
- Colour Index
- 45005 · 45005
- color
- Pink
- Water Solubility
- Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
- λmax
- 548 nm · 548 nm
- Merck
- 13 · 13,8097
- BRN
- 3795789 · 3795789
- Stability
- Stable. Incompatible with strong oxidizing agents.
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
GHS classification
GHS Classification
Warning
H341 (100%): Suspected of causing genetic defects [Warning Germ cell mutagenicity]
P203, P280, P318, P405, and P501 (click each P-code to see the statement)
The GHS information provided by 1 company from 1 notification to the ECHA C&L Inventory.
Hazard information
Regulatory Information
Chemical: Methanaminium, N-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methyl-, chloride
Xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1) is listed on the EPA's Chemical Data Reporting (CDR) system. Manufacturers and importers of Xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1) are required to report information about their production and use of this chemical to the EPA under the Toxic Substances Control Act (TSCA). (40 eCFR Part 711)
Xanthylium, 3,6-bis(dimethylamino)-, chloride: Does not have an individual approval but may be used under an appropriate group standard
Hazards SummaryCauses changes in kidneys (acute tubular necrosis) in intravenous lethal-dose studies of mice (LD50 = 179 mg/kg); [RTECS] A skin and strong eye irritant; Targets the kidney and bladder; [Amresco MSDS]
Hazard Classes and CategoriesMuta. 2 (100%)
Safety data
- PPE
- Eyeshields, Gloves, type N95 (US)
- wgk
- 3
- tsca
- TSCA listed
- rtecs
- BQ1450000
- hs_code
- 32041300
- risk_code
- 68-36/37/38
Chemistry & industry
Pharmacology
MeSH Pharmacological ClassificationChemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
Uses & manufacturing
UsesUsed as a bacterial and biological stain; [Merck Index]
U.S. Production2022: 11,116 lb
Industry UsesFiller
General Manufacturing Information
All Other Chemical Product and Preparation Manufacturing
Xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1): ACTIVE
Applications
UsesPyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
UsesPyronin Y has been used: in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)staining of CD34+ cells
UsesBacterial and biological stain.
General DescriptionPyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.
Suppliers100 total
Related reactions
0 records
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Compound notes
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