Lysyllysyllysine

UsesRecommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.

UsesPoly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. It has also been used as a transfection agent for efficient labeling and to facilitate endocytosis. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

UsesPoly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.

General DescriptionPoly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.