Basic information
- CAS
4264-83-9- Standard SMILES
O=[N+]([O-])c1ccc(OP(=O)([O-])[O-])cc1.[Na+].[Na+]- InChIKey
VIYFPAMJCJLZKD-UHFFFAOYSA-L- Molecular formula
- C6H4NNa2O6P
- Average molar mass
- 263.05 g/mol
- Monoisotopic mass
- 262.95716241
Names & identifiers
- Preferred name
- Disodium 4-nitrophenyl phosphate
- IUPAC
- disodium;(4-nitrophenyl) phosphate
- English name
- Disodium 4-nitrophenylphosphate
- Molecular formula
- C6H7NNaO6P
- Molecular weight
- 243.09
Names & synonyms
Disodium 4-nitrophenyl phosphatedisodium p-nitrophenyl phosphatePhosphoric acid, mono(4-nitrophenyl) ester, disodium saltEINECS 224-246-5Phosphoric acid, mono(4-nitrophenyl) ester, sodium salt (1:2)Phosphoric acid, mono(p-nitrophenyl) ester, disodium saltRefChem:589385diSodium 4nitrophenyl phosphatePhosphoric acid, mono(4nitrophenyl) ester, disodium saltPhosphoric acid, mono(pnitrophenyl) ester, disodium saltPhosphoric acid, mono(4nitrophenyl) ester, sodium salt (1:2)224-246-5
Show more names
PNPP4-NPP4-PNPPABTS<PNPP-NA2PNPP, 2NA2-oxazolePNPP di-NAPNP-PHOS DINA
4264-83-9Disodium 4-Nitrophenylphosphate4-nitrophenyl phosphate disodium saltSodium 4-nitrophenyl phosphatePNPP DisodiumC6H4NNa2O6Pdisodium;(4-nitrophenyl) phosphateMFCD00007319
Database identifiers
Properties
Computed structural properties
- Topological polar surface area
- 118 Ų
- Hydrogen bond donors
- 0
- Hydrogen bond acceptors
- 6
- Rotatable bonds
- 1
- Heavy atoms
- 16
- Formal charge
- 0
- Complexity
- 238
Experimental & physicochemical properties
Chemical ClassesNitrogen Compounds -> Nitrophenols
Physical DescriptionHexahydrate: Solid; [Sigma-Aldrich MSDS]
Physicochemical properties
- Melting point
- 300 °C · >300 °C(lit.)
- Density
- 1.751 at · 1.751 at 20℃
- vapor pressure
- 15 Pa · 15Pa at 20-50℃
- storage temp.
- -20 °C · -20°C
- solubility
- 2 O: · H2O: 1 tablet/3 mL 25 mM, clear, light yellow
- form
- tablets
- color
- white to pale yellow
- Water Solubility
- Miscible with water. Immiscible with ethanol and acetone.
- BRN
- 5324661 · 5324661
- Stability
- Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.
- InChI
- 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
Safety & regulation
Safety information is for reference only and does not replace the supplier’s Safety Data Sheet (SDS), applicable regulations, or professional assessment.
GHS classification
GHS Classification
This chemical does not meet GHS hazard criteria for 40% (2 of 5) of reports.
Warning
H302 (60%): Harmful if swallowed [Warning Acute toxicity, oral]
P264, P270, P301+P317, P330, and P501 (click each P-code to see the statement)
Aggregated GHS information provided per 5 reports by companies from 2 notifications to the ECHA C&L Inventory.
Hazard information
Regulatory Information
Chemical: Phosphoric acid, mono(4-nitrophenyl) ester, disodium salt
REACH registered substance: Disodium 4-nitrophenyl phosphate (EC: 224-246-5)
Phosphoric acid, mono(4-nitrophenyl) ester, disodium salt: Does not have an individual approval but may be used under an appropriate group standard
Hazards SummaryMay cause irritation; [Sigma-Aldrich MSDS] See 4-Nitrophenol.
Hazard Classes and CategoriesAcute Tox. 4 (60%)
Safety data
- F
- 10-21
- wgk
- 3
- tsca
- TSCA listed
- hs_code
- 29199000
- risk_code
- 36/38-36/37/38-48/22-41-38-21/22
- Signal word
- Danger
- Symbol(GHS)
- GHS05,GHS08
- hazard_code
- Xi,Xn
- safety_code
- 26-36-24/25-23-36/37/39
- Storage Class
Chemistry & industry
Uses & manufacturing
General Manufacturing InformationPhosphoric acid, mono(4-nitrophenyl) ester, sodium salt (1:2): ACTIVE
Applications
UsesPNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures.
General DescriptionExcellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.
Upstream & downstream products
Upstream raw materials
Suppliers100 total
Related reactions
0 records
No reaction records under the current filter.
Compound notes
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