Biotin hydrazide

DescriptionBiotin hydrazide is a biotinyl derivative used to label surface functional groups, antibodies, lectins, sugars, nucleic acids, or molecules with free carboxylic or keto groups. Biotin hydrazide is used as a probe for determining protein carbonylation, an irreversible posttranslational modification resulting from the actions of reactive oxygen species or lipid oxidation products. The reaction of this probe is direct and does not require catalysts or reducing agents.

UsesBiotin Hydrazide is a carbohydrate reactive biotinylation reagent used predominately for labeling monoclonal antibodies, unpaired cytosine residues in nucleic acids and glycoproteins.

Usesaldehyde reactive biotinylation reagent

Uses(+)-Biotin hydrazide has been used:the modification of alginatefor the labelling of mitochondrial proteins from non-muscle tissues as a component of glycoprotein staining solutionin periodic acid-biotin-hydrazide (PABH) assay for mucinsfor labeling surface functional groups, biologically active molecules such as antibodies, lectins, sugars, nucleic acids or molecules with free carboxylic or keto groups.for coupling to glycoproteins through the carbohydrate by hydrazone formation

General DescriptionBiotin hydrazide is a biotinylation reagent used to biotinylate glycoproteins with their sugar moieties. Biotin hydrazide can be used to prepare biotin-conjugated alginate. It can also be used for covalent attachment to PAAc via carbodi-imide cross linking.