D-(-)-Fructose → GlyTouCan:G36918VO

Participants

Reaction Steps

Specifically, an isomaltooligosaccharide saccharification solution with Bx of 70 to 73 was obtained by adding water to a liquid fructose (HFCS-42, Ingredion Korea) having a fructose content of 42 to 45% (w/w) based on solids and Bx of 75 to 77. To the solution was added a dextrose saccharification enzyme, glucoamylase (AMG, Novozymes Korea), in an amount of 2.0 to 2.5% (w/w) based on the total weight of solids in the saccharification solution at a temperature of 65 to 70° C. and a pH of 4.3 to 4.7, followed by a reaction for 90 to 96 hours. The obtained solution was subject to filtration using diatomite to remove the unreacted materials, decolorization by passing through a column filled with granular active carbon at a temperature of 70 to 75° C., ion purification using a cation exchange resin (PK218, Samyang) and an anion exchange resin (WA30, Samyang), and evaporation to remove moisture to obtain an isomaltulose-containing isomaltooligosaccharide composition with Bx of 75 to 77.

Provenance & Evidence