Phosphoenolpyruvate → L-Glutamic Acid

Reaction equation

C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O.C=C(OP(O)(O)=O)C(O)=O.[NH2:24][C@H:25]([C:31]([O-:33])=[O:32])[CH2:26][CH2:27][C:28]([O-:30])=[O:29]>>[NH2:24][C@H:25]([C:31]([OH:33])=[O:32])[CH2:26][CH2:27][C:28]([OH:30])=[O:29]

Participants

Reactant 3

HCID 1005Phosphoenolpyruvate

HCID 31348Citrate

HCID 5460544L-glutamate(2-)

Product 1

HCID 33032L-Glutamic Acid

Procedure

Subsequently, the citrate synthase gene, phosphoenolpyruvate carboxylase gene and glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

Workup

additionwere introduced into the SC17sucA strain

Sources & evidence

ORD record: ord-a8157e506ba647088ae91dc2f0088eb6
Source dataset: uspto-grants-2010_10
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: USRE041800E1

Reaction notes

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