Phosphoenolpyruvate → L-Glutamic Acid

Reaction equation

C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O.C=C(OP(O)(O)=O)C(O)=O.[NH2:24][C@H:25]([C:31]([O-:33])=[O:32])[CH2:26][CH2:27][C:28]([O-:30])=[O:29]>>[NH2:24][C@H:25]([C:31]([OH:33])=[O:32])[CH2:26][CH2:27][C:28]([OH:30])=[O:29]

Participants

Reactant 3

HCID 1005Phosphoenolpyruvate

HCID 31348Citrate

HCID 5460544L-glutamate(2-)

Product 1

HCID 33032L-Glutamic Acid

Procedure

Subsequently, the citrate synthase gene, phosphoenolpyruvate carboxylase gene and glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

Workup

additionwere introduced into the SC17sucA strain

Sources & evidence

ORD record: ord-58ceeb54159540d89c15caf417f52855
Source dataset: uspto-grants-2007_04
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07198943B2

Reaction notes

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