Sodium Chloride → HCID 178446018, compound

HRID 127577

Reaction equation

HRID 127577 reaction equation
C([NH:13][C@H:14]([C:21]([NH:23][C@H:24]([C:29]([OH:31])=[O:30])[CH2:25][CH:26]([CH3:28])[CH3:27])=[O:22])[CH2:15][C:16]1[N:20]=[CH:19][NH:18][CH:17]=1)(=O)CNC(C1C=CC=CC=1)=O.[Cl-].[Na+].C[C@H](NC([C@H]1N(C([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](N)CC(O)=O)=O)CCCNC(N)=N)=O)C(C)C)=O)CC2C=CC(O)=CC=2)=O)C(C)C)=O)CC2NC=NC=2)=O)CCC1)=O)C(O)=O>O>[NH2:13][C@H:14]([C:21]([NH:23][C@H:24]([C:29]([OH:31])=[O:30])[CH2:25][CH:26]([CH3:27])[CH3:28])=[O:22])[CH2:15][C:16]1[N:20]=[CH:19][NH:18][CH:17]=1

Participants

5

Reactant 3

HCID 5234Sodium ChlorideHCID 178445996HCID 178445996, compoundHCID 178448346H-Asp-Arg-Val-Tyr-Val-His-Pro-Ala-OH

Product 1

HCID 178446018HCID 178446018, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

50 μl of a solution containing 0.01 mole/liter of hippuryl-histidyl-leucine (substrate) and 0-100 μl of a test compound solution were added to 300 μl of a 0.2 M trishydrochloric acid buffer solution containing 0.2 mole/liter of sodium chloride. The total volume of said mixture was adjusted to 450 μl with water. Then, 50 μl of angiotensin-converting enzyme (ACE) isolated from pig's renal cortex were added to the mixture, and the mixture was allowed to stand at 37° C. for 20 minutes. The amount of histidyl-leucine produced from the substrate by the action of ACE was assayed microbiologically by the use of Leuconostoc mesenteroides P-60, and the ACE-inhibitory activity of the test compound was estimated therefrom.

Workup

  1. customisolated from pig's renal cortex
  2. additionwere added to the mixture

Sources & evidence

ORD record
ord-9d8eac79c8ca4160973c44fa1d2bf8e0
Source dataset
uspto-grants-1981_03
Patent number
US04256751

Linked notes

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