SK&F 81297 → Dopamine

HRID 1468604

Reaction equation

HRID 1468604 reaction equation
CCCN1[C@@H]2CC3C4C(=CC=CC=4NC=3)[C@H]2C[C@@H](CSC)C1.C1[C:33]2[C:28](=[CH:29][C:30]([OH:36])=[C:31]([OH:35])[C:32]=2Cl)[CH:27](C2C=CC=CC=2)[CH2:26][NH:25]C1>>[NH2:25][CH2:26][CH2:27][C:28]1[CH:33]=[CH:32][C:31]([OH:35])=[C:30]([OH:36])[CH:29]=1

Participants

3

Reactant 2

HCID 1218SK&F 81297HCID 47811Pergolide

Product 1

HCID 681Dopamine

Procedure

HEK cells were transfected with a DNA construct encoding D1-NLS-GFP (1.5 micrograms of DNA), and incubated with the agonist pergolide (10 micromolar) for 48 hours. At 6, 22, 30 and 42 hours after transfection, the cells were treated with fresh medium containing SKF-81297 (final concentration 10 micromolar). The cells were examined by confocal microscopy.

Workup

  1. additionAt 6, 22, 30 and 42 hours after transfection, the cells were treated with fresh medium

Sources & evidence

ORD record
ord-eafaf474af62467882714ab53dff96ee
Source dataset
uspto-grants-2015_04
Patent number
US09005905B2

Linked notes

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