Isoflurane → Fluasterone

HRID 1477074

Reaction equation

HRID 1477074 reaction equation
C(Cl)(OC(F)F)C(F)(F)F.CNC1(C2C=CC=CC=2Cl)C(=O)CCCC1.CC1C=CC=C(C)C=1NC1SCCCN=1.CN1[C@@H]2C[C@@H](OC(C(C3C=CC=CC=3)CO)=O)C[C@H]1CC2.[CH3:63][C@@:64]12[C@H:73]3[CH2:74][CH2:75][C@:76]4([CH3:83])[C:80](=[O:81])[C@H:79]([F:82])[CH2:78][C@H:77]4[C@@H:72]3[CH2:71][CH:70]=[C:69]1[CH2:68][CH2:67][CH2:66][CH2:65]2.C(Cl)C(OC(N)=O)CCl>CC(C)=O>[CH3:63][C@@:64]12[C@H:73]3[CH2:74][CH2:75][C@:76]4([CH3:83])[C:80](=[O:81])[C@H:79]([F:82])[CH2:78][C@H:77]4[C@@H:72]3[CH2:71][CH:70]=[C:69]1[CH2:68][CH2:67][CH2:66][CH2:65]2

Participants

7

Reactant 5

HCID 3763IsofluraneHCID 3821KetamineHCID 5707XylazineHCID 131736474fluasterone Cremophor EL3×HCID 178481186HCID 178481186, compound

Product 1

HCID 133967Fluasterone

Reagents, catalysts & solvents 1

HCID 180AcetoneSolvent

Procedure

The mice were anesthetized with Isoflurane, and then were given an injection of 0.1 mL solution of ketamine (50 mg/kg), xylazine (1.0 mg/kg) and atropine (0.1 mg/kg). Approximately 10 minutes after the i.m. injection (after the mice could not turn over when placed on their backs), the mice were treated with 3.8 μL of a 12.5 mg/mL fluasterone/Cremophor EL solution to give a dose of 1.9 mg/kg. The mice were anesthetized and on their backs for approximately 30 minutes after application of fluasterone/Cremophor EL solution. One hour after the application of fluasterone/Cremophor EL solution, 2 μg of TPA dissolved in 0.2 mL of acetone was applied to the shaved area on the back of each mouse.

Workup

  1. customApproximately 10 minutes
  2. customto give a dose of 1.9 mg/kg

Sources & evidence

ORD record
ord-5752513c11df42b3bd87238f14fc8ad7
Source dataset
uspto-grants-2016_08
Patent number
US09402853B2

Linked notes

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