L-Glutamic Acid → L-Glutamine

HRID 1509799

Reaction equation

HRID 1509799 reaction equation
[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][C:5]([NH:7][CH2:8][CH3:9])=[O:6].[NH2:13][C@H:14]([C:20]([OH:22])=[O:21])[CH2:15][CH2:16][C:17]([OH:19])=[O:18]>>[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][C:5](=[O:6])[NH2:7].[NH2:1][C@H:2]([C:10]([OH:12])=[O:11])[CH2:3][CH2:4][C:5]([NH:7][CH2:8][CH3:9])=[O:6].[NH2:13][C@H:14]([C:20]([OH:22])=[O:21])[CH2:15][CH2:16][C:17]([OH:19])=[O:18]

Participants

5

Reactant 2

HCID 33032L-Glutamic AcidHCID 439378L-Theanine2×

Product 3

HCID 5961L-GlutamineHCID 33032L-Glutamic AcidHCID 439378L-Theanine

Procedure

An enzyme reaction liquid in which theanine enzyme synthesis was executed was diluted suitably and thereafter, HPLC was carried out for the diluted reaction liquid so that amounts of theanine and glutamic acid were determined. A mol conversion ratio from an amount of glutamine (mol/L) of the substrate was calculated using obtained amounts of theanine and glutamic acid (mol/L). TABLE 1 shows the conditions for determination by HPLC.

Workup

  1. customsynthesis
  2. additionwas executed was diluted suitably

Sources & evidence

ORD record
ord-36e9f754a31f4801b2a657445eb30933
Source dataset
uspto-grants-2012_07
Patent number
US08211674B2

Linked notes

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