Dianisidine → 2-Naphthol

Participants

Reaction Steps

Silica particles were derivatized with o-dianisidine by Method No. 2. After drying, 1.0 gm of the carrier was diazotized with sodium nitrite. After diazotization the derivative was washed several times with borate (0.03M) buffered saline (0.15M) pH 8.0. The derivative was then reacted with 1.5 ml of T4 antisera (titer 1:10000) at 4°C. with stirring. The pH was maintained between 8.0 - 8.5 with 0.1N NaOH. After 30 minutes the pH stabilized at 8.2 and the reaction was stirred slowly at 4°C. overnight. After this time the composite gave a negative β-napthol test indicating no more remaining functional diazo groups. The preparation was then washed (4X) with phosphate-buffer (0.03M) saline (0.15M) containing 0.1% bovine serum albumin (PBS-BSA).

Provenance & Evidence