(+)-Lactose → D-Galactose

HRID 1597767

Reaction equation

HRID 1597767 reaction equation
OC1O[C@H](CO)[C@@H]([O:8][C@@H:9]2[O:17][C@H:16]([CH2:18][OH:19])[C@H:14]([OH:15])[C@H:12]([OH:13])[C@H:10]2[OH:11])[C@H](O)[C@H]1O.CC(N[C@H]1[C@@H](OP(OP(OC[C@H]2O[C@@H](N3C(=O)NC(=O)C=C3)[C@H](O)[C@@H]2O)(O)=O)(O)=O)O[C@H](CO)[C@@H](O)[C@@H]1O)=O>>[OH:8][CH:9]1[O:17][C@H:16]([CH2:18][OH:19])[C@H:14]([OH:15])[C@H:12]([OH:13])[C@H:10]1[OH:11]

Participants

3

Reactant 2

HCID 440995(+)-LactoseHCID 445675Uridine-Diphosphate-N-Acetylglucosamine

Product 1

HCID 6036D-Galactose

Procedure

Lactose was contacted with UDP-N-acetylglucosamine and a β-galactoside β1-3 N-acetylglucosaminyl transferase purified from pig serum in a 0.1M HEPS buffered aqueous solution at 37° C. The product tetrasaccharide was then contacted with UDP--Gal and a β-N-acetylglucosaminoside β1-4 Galactosyltransferase isolated from bovine milk, in a 0.1M HEPS buffered aqueous solution at 37° C. The product tetrasaccharide was then contacted with CMP--NAN and a β-galactoside α2-6 sialyltransferase isolated from rat liver in a 0.1M HEPES buffered aqueous solution at 37° C. The title pentasaccharide was isolated by conventional methods.

Workup

  1. customa β-galactoside β1-3 N-acetylglucosaminyl transferase purified from pig serum in a 0.1M HEPS
  2. customat 37° C

Sources & evidence

ORD record
ord-2aad3994a43944f89f6c9c7ec6c9ee71
Source dataset
uspto-grants-1998_04
Patent number
US05736533

Linked notes

Loading…