L-Glutamine → L-Alanyl-L-Glutamine

HRID 16050

Reaction equation

HRID 16050 reaction equation
Cl.C[O:3][C:4](=O)[C@H:5]([CH3:7])[NH2:6].[NH2:9][C@H:10]([C:16]([OH:18])=[O:17])[CH2:11][CH2:12][C:13](=[O:15])[NH2:14].C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O>B([O-])([O-])[O-]>[NH2:6][C@H:5]([C:4]([NH:9][C@H:10]([C:16]([OH:18])=[O:17])[CH2:11][CH2:12][C:13](=[O:15])[NH2:14])=[O:3])[CH3:7]

Participants

5

Reactant 3

HCID 5961L-GlutamineHCID 6049Ethylenediaminetetraacetic AcidHCID 2733257Alanine methyl ester hydrochloride

Product 1

HCID 123935L-Alanyl-L-Glutamine

Reagents, catalysts & solvents 1

HCID 26574BorateSolvent

Procedure

3 μl of the same enzyme fraction as used in Example 5 was added to 100 μl of 100 mM borate buffer (pH 9.0) containing 100 mM L-alanine methyl ester hydrochloride, 200 mM L-glutamine and 10 mM EDTA, and allowed to react at 18° C. As a result, as shown in FIG. 3, 83 mM L-alanyl-L-glutamine (L-Ala-L-Gln) was formed in the case of an enzyme-added lot, and the concentration of by-product L-Ala-L-Ala-L-Gln was 1.3 mM. On the other hand, there was scarcely any production of L-Ala-L-Gln observed in an enzyme-non-added lot, and the enzyme concentration was only about 0.07 mM after reacting for 120 minutes.

Workup

  1. customto react at 18° C

Sources & evidence

ORD record
ord-1b4406b554174c0997e716b5b5502a8a
Source dataset
uspto-grants-2010_06
Patent number
US07736876B2

Linked notes

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