Kaempferol → Ipriflavone

HRID 1622073

Reaction equation

HRID 1622073 reaction equation
[Ca].[CH:2]1[C:7]([C:8]2[O:18][C:17]3[CH:16]=[C:15]([OH:19])[CH:14]=[C:13]([OH:20])[C:12]=3[C:10](=[O:11])[C:9]=2[OH:21])=[CH:6][CH:5]=[C:4]([OH:22])[CH:3]=1>>[CH:2]1[C:7]([C:8]2[O:18][C:17]3[CH:16]=[C:15]([OH:19])[CH:14]=[C:13]([OH:20])[C:12]=3[C:10](=[O:11])[C:9]=2[OH:21])=[CH:6][CH:5]=[C:4]([OH:22])[CH:3]=1.[CH3:8][CH:9]([O:19][C:15]1[CH:14]=[CH:13][C:12]2[C:10](=[O:11])[C:9]([C:2]3[CH:3]=[CH:4][CH:5]=[CH:6][CH:7]=3)=[CH:8][O:18][C:17]=2[CH:16]=1)[CH3:10]

Participants

4

Reactant 2

HCID 5280863KaempferolHCID 5460341Calcium3×

Product 2

HCID 3747IpriflavoneHCID 5280863Kaempferol

Procedure

The enhancement of calcium deposition between kaempferol and ipripfavone were compared. Test samples and their adding manner were same as Experiment 4. Calcium deposition amount was measured by usual method using “Calcium C Test Wako” (commercialized by Wako Pure Chemical Industries, Osaka, Japan); briefly, cultured cells were washed with 1 ml of Dulbecco phosphate buffer saline three times after removing the medium. To the resultant was added 0.5 ml of 2N hydrochloric acid to resolve deposited calcium. To 5 μl of the resolving solution were added 0.5 ml of 0.88M monoethanolamine buffer (pH11) and 0.05 ml of 0.63 mM o-cresolphthalein complexone solution (binding to calcium ion to form chelate complex) containing 69 mM 8-quinolinol and mixed, Calcium amount per one hole of the plate was calculated by measuring absorbance 570 nm of the resultants and standardizing with a standard calcium solution. The result of comparison with kaempferol and ipriflavone was shown in FIG. 2. The result of only ethanol treatment as a negative control is also shown in FIG. 2.

Workup

  1. additionTest samples and their adding manner
  2. washbriefly, cultured cells were washed with 1 ml of Dulbecco phosphate buffer saline three times
  3. customafter removing the medium
  4. additionTo the resultant was added 0.5 ml of 2N hydrochloric acid
  5. additionTo 5 μl of the resolving solution were added 0.5 ml of 0.88M monoethanolamine buffer (pH11) and 0.05 ml of 0.63 mM o-cresolphthalein complexone solution (binding to calcium ion to form chelate complex)
  6. additioncontaining 69 mM 8-quinolinol
  7. additionmixed

Sources & evidence

ORD record
ord-c6608fcccc1149f7a043a18e9ffbbc50
Source dataset
uspto-grants-2014_07
Patent number
US08778882B2

Linked notes

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