Sodium Chloride → 2'-Deoxycytidine-5'-Monophosphate
Reaction equation
C1[C@H](N2C3NC=NC(=O)C=3N=C2)O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@H]1O.[P:31]([O:43][CH2:44][C@H:45]1[O:49][C@@H:48]([N:50]2[CH:57]=[CH:56][C:54]([NH2:55])=[N:53][C:51]2=[O:52])[CH2:47][C@@H:46]1[OH:58])([O:34]P(OP(O)(O)=O)(O)=O)(=[O:33])[OH:32].P([O-])([O-])([O-])=O.[K+].[K+].[K+].[Cl-].[Mg+2].[Cl-].[Cl-].[Na+].C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O.[Na+].[Na+].[Na+]>>[CH2:47]1[C@H:48]([N:50]2[C:51](=[O:52])[N:53]=[C:54]([NH2:55])[CH:56]=[CH:57]2)[O:49][C@H:45]([CH2:44][O:43][P:31]([OH:33])([OH:34])=[O:32])[C@H:46]1[OH:58]Participants
Conditions
- Temperature
- -20 °C
Procedure
A reaction mixture for the synthesis of poly (dI:dC) contains 3.5 μ moles each of dITP and deoxycytidine-5'-triphosphate (dCTP), 600 μ moles of potassium phosphate buffer (pH 7.4) and 30 μ moles of magnesium chloride, and 40-80 units of DNA (deoxynucleic acid) polymerase in a total volume of 10 ml. After a lag period of 2-4 hours, polymerization is complete in 6-8 hours. The course of the reaction is followed by optical density measurements at intervals. When hypochromicity at 260 mμ is at a maximum, the reaction is stopped by adjusting the mixture to 0.2M sodium chloride and 0.1M sodium citrate. Dialysis is extensively carried out against the same buffer to remove all low molecular weight material. The product is stored frozen at -20° C. until used for interferon induction.
Workup
- customA reaction mixture for the synthesis of poly (dI:dC)
- customAfter a lag period of 2-4 hours, polymerization
- customDialysis
- customto remove all low molecular weight material
Sources & evidence
- ORD record
- ord-13ca69f532d84eb8b6efb15825b8ecb0
- Source dataset
- uspto-grants-1978_11
- Patent number
- US04124702
Reaction notes
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