Sodium Carbonate → Biotin

HRID 1748841

Reaction equation

HRID 1748841 reaction equation
[CH2:1]1[S:5][C@@H:4]([CH2:6][CH2:7][CH2:8][CH2:9][C:10](NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=[O:11])[C@H:3]2[NH:32][C:33]([NH:35][C@@H:2]12)=[O:34].[Na+].C(=O)([O-])[O-:38].[Na+].[Na+]>>[OH:38][C:10]([CH2:9][CH2:8][CH2:7][CH2:6][C@H:4]1[C@@H:3]2[C@@H:2]([NH:35][C:33]([NH:32]2)=[O:34])[CH2:1][S:5]1)=[O:11]

Participants

3

Reactant 2

HCID 10340Sodium CarbonateHCID 71571497Sulphosuccinimidyl-6-(biotinamido)hexanoate

Product 1

HCID 171548Biotin

Procedure

The RA-NAH (10 mg) obtained in the above was dissolved in 0.5 ml of 0.1 M sodium carbonate aqueous solution, subsequently adding 3 mg of sulfo-NHS-LC-biotin (mfd, by Pierce Chemical Company) thereto followed by overnight incubation at 4° C. After overnight dialysis against distilled water thereafter, the dialyzed liquid was lyophilized to obtain the polysaccharide of the present invention in which biotin is bound to the reducing end of NAH (to be referred to as “NAH-biotin” hereinafter) (8 mg).

Workup

  1. customThe RA-NAH (10 mg) obtained in the
  2. customat 4° C
  3. customAfter overnight dialysis
  4. distillationagainst distilled water

Sources & evidence

ORD record
ord-1b26caafa6a644a48874c7472e44e884
Source dataset
uspto-grants-2015_10
Patent number
US09150902B2

Linked notes

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