SK&F 81297 → Dopamine

HRID 2068455Notes

Reaction equation

HRID 2068455 reaction equation
CCCN1[C@@H]2CC3C4C(=CC=CC=4NC=3)[C@H]2C[C@@H](CSC)C1.C1[C:33]2[C:28](=[CH:29][C:30]([OH:36])=[C:31]([OH:35])[C:32]=2Cl)[CH:27](C2C=CC=CC=2)[CH2:26][NH:25]C1>>[NH2:25][CH2:26][CH2:27][C:28]1[CH:33]=[CH:32][C:31]([OH:35])=[C:30]([OH:36])[CH:29]=1

Participants

3

Reactant 2

HCID 1218SK&F 81297HCID 47811Pergolide

Product 1

HCID 681Dopamine

Procedure

HEK cells were transfected with a DNA construct encoding D1-NLS-GFP (1.5 micrograms of DNA), and incubated with the agonist pergolide (10 micromolar) for 48 hours. At 6, 22, 30 and 42 hours after transfection, the cells were treated with fresh medium containing SKF-81297 (final concentration 10 micromolar). The cells were examined by confocal microscopy.

Workup

  1. additionAt 6, 22, 30 and 42 hours after transfection, the cells were treated with fresh medium

Sources & evidence

ORD record
ord-688973ee7bcc4281899a9883ffcfc926
Source dataset
uspto-grants-2012_10
Patent number
US08298777B2

Reaction notes

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