X-gal NMP methanol → NMP Methanol

HRID 2169657

Reaction equation

HRID 2169657 reaction equation
C1C2NC=[C:9]([O:10][C@@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)C=2C(Cl)=C(Br)C=1.[CH3:24][N:25]1[C:29](=[O:30])[CH2:28][CH2:27][CH2:26]1.CO>C1C2NC=C(O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)C=2C(Cl)=C(Br)C=1>[CH3:24][N:25]1[C:29](=[O:30])[CH2:28][CH2:27][CH2:26]1.[CH3:9][OH:10]

Participants

3

Reactant 1

HCID 131730744X-gal NMP methanol

Product 1

HCID 22395687NMP Methanol

Reagents, catalysts & solvents 1

HCID 65181X-GalSolvent

Procedure

When the 1× X-gal/NMP/methanol is stored for 16 weeks at 4° C., there is no loss in X-gal activity as measured by applying 100 μl to a LB plate, streaking E. coli pUC19 transfectant, incubation at 37° C. and examination for blue colonies after 16 hours.

Sources & evidence

ORD record
ord-27fdc3d795a844069d6956acf50b094a
Source dataset
uspto-grants-2015_06
Patent number
US09046495B2

Linked notes

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