Citric Acid → 4-Methylumbelliferone

HRID 244286

Reaction equation

HRID 244286 reaction equation
[C:1]([OH:13])(=O)[CH2:2][C:3]([CH2:8][C:9]([OH:11])=O)([C:5](O)=O)O.[OH2:14]>>[CH3:5][C:3]1[C:2]2[CH:1]=[CH:2][C:3]([OH:14])=[CH:5][C:1]=2[O:13][C:9](=[O:11])[CH:8]=1

Participants

3

Reactant 2

HCID 311Citric AcidHCID 962Water

Product 1

HCID 52805674-Methylumbelliferone

Procedure

An enzymatic reaction was initiated by the following steps under 25° C. condition: adding to a 96-well microplate 50 μl of a 4-MUO buffer solution and 25 μl of distilled water (or an aqueous sample solution) for each well; mixing them; and then adding 25 μl of a lipase buffer solution to each well. After a 30-minute reaction, 100 μl of a 0.1M citric acid buffer (pH4.2) was added to terminate the reaction, and the fluorescence of 4-methylumbelliferone (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) produced by the reaction was measured with a fluorescence plate reader (Fluoroskan Asent CF from Labsystems, Inc.)

Workup

  1. customAn enzymatic reaction
  2. customwas initiated by the following steps under 25° C.
  3. additionmixing them
  4. additionwas added
  5. customto terminate
  6. customthe reaction

Sources & evidence

ORD record
ord-7a9ac03512844c1e9b9a4b04c066e695
Source dataset
uspto-grants-2014_02
Patent number
US08658237B2

Linked notes

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