Thymidine → Deoxyuridine

Participants

Reaction Steps

Strain CMG1115 (see Table 4 above) can be modified to a genotype additionally including tdk+ (thymidine kinase) and thyA- (thymidylate synthase negative) in order to produce deoxyuridine in accordance with the present invention. Such a modified strain derived from strain CMG1115 (containing pCG144), is streaked on LB agar plates with 100 ug/mL ampicillin and 50 mg/ml thymidine and grown at 30° C. Single colonies are picked into 25 mL of LB broth with 100 μg/ml ampicillin in a 250 mL baffle flask and incubated at 30° C. for 8 hours. This culture (0.5 mL) is then used to seed flasks with production media with the composition presented in Table 11.

Provenance & Evidence