NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

Participants

Reaction Steps

To 2.5 ml of Tris-imidazole-sodium acetate buffer solution (solution containing 0.12 M each is adjusted to pH of 9.5 with 4 N NaOH) is added 0.2 ml of 15 mM NADP+ solution After keeping at 37° C. for 5 minutes, 0.1 ml of an enzyme solution is added and 0.2 ml of 150 mM L-fucose solution is further added to the system to initiate the reaction. Immediately thereafter, the reaction mixture is transferred to a cell for measurement of absorbance (1 cm light path) kept at 37° C. and absorbance is measured at the wavelength of 340 nm for 2 minutes or, if necessary, over a longer time period. One unit is an amount of enzyme that produces 1 μmole of NADPH for one minute.

Provenance & Evidence