Phosphate → D-Glucosamine

Participants

Reaction Steps

The activity is determined by the following method. Namely, 0.3 ml of a 10% solution of N-acetyl-D-glucosamine is added as a substrate to 0.1 ml of a 200 mM phosphate buffer solution (pH 7.8). Next, 0.1 ml of an enzyme solution is further added thereto followed by incubation at 48° C. for 30 minutes. Then the D-glucosamine thus formed is determined by the indole/hydrochloric acid colorimetry. The amount of the enzyme by which 1 μmol of D-glucosamine is formed in a minute is referred to as 1 U (unit).

Provenance & Evidence