Citric Acid → Thromboxane

HRID 280845

Reaction equation

HRID 280845 reaction equation
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][C@H:6](O)/[CH:7]=[CH:8]/[C@@H:9]1[C@@H:13]([CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH2:18][CH2:19][C:20](O)=O)[C@H:12]2O[O:24][C@@H:10]1[CH2:11]2.C(O)(=O)CC(CC(O)=O)(C(O)=O)O>CCOCC>[CH3:20][CH2:19][CH2:18][CH2:17][CH2:16][CH2:15][CH2:14][C@@H:13]1[C@@H:9]([CH2:8][CH2:7][CH2:6][CH2:5][CH2:4][CH2:3][CH2:2][CH3:1])[O:24][CH2:10][CH2:11][CH2:12]1

Participants

4

Reactant 2

HCID 311Citric AcidHCID 445049Prostaglandin H2

Product 1

HCID 114873Thromboxane

Reagents, catalysts & solvents 1

HCID 3283Diethyl EtherSolvent

Procedure

To a 12×75 mm tube containing [1-14C] PGH2 (2 μg, 0.01 μCi or 0.05 μCi) and inhibitor (1 mM final assay concentration, in 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung microsomes (25 μl, containing 400 μg protein) or an aliquot of human platelet microsomes (25 μl, containing ≅130 g protein). The solution was incubated in a 22° water bath for 2 min.; ether (1 ml) and citric acid (25 μMoles) were added, and the tube plunged into an acetone-dry ice bath. The ether extract was transferred to a 1.0 ml Reacti-vial, and the volume reduced under nitrogen to ≅50 μl.

Workup

  1. concentrationassay concentration
  2. additionin 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung microsomes (25 μl, containing 400 μg protein)
  3. additionan aliquot of human platelet microsomes (25 μl, containing ≅130 g protein)
  4. customwas incubated in a 22°
  5. customfor 2 min.
  6. customthe tube plunged into an acetone-dry ice bath
  7. extractionThe ether extract

Sources & evidence

ORD record
ord-3fb143d178f5413aa787aa30852b9720
Source dataset
uspto-grants-1986_05
Patent number
US04591594

Linked notes

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