Kanamycin → L-Phenylalanine

Participants

Role	Name	HCID	Charge
Reactant	Kanamycin	HCID 6032	—
Reactant	L-Leucine	HCID 6106	—
Reactant	Trisodium citrate	HCID 6224	—
Reactant	Potassium Phosphate, Dibasic	HCID 24450	—
Reactant	(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal	HCID 107526	—
Reactant	Monobasic potassium phosphate	HCID 516951	—
Reactant	HCID 178418600	HCID 178418600	—
Product	L-Phenylalanine	HCID 6140	—

Reaction Steps

The cell-suspension was transferred onto a minimum medium prepared by adding 5 μg/dl kanamycin, 10 mg/dl L-leucine, 10 mg/dl L-leucine, 10 mg/dl L-arginine, 100 mg/dl D, L-m-fluorophenylalanine and 2 g/dl agar to a basal minimum medium of pH 7.2 containing 0.6 g/dl KH2PO4, 1.4 g/dl K2HPO4, 0.2 g/dl (NH4)2SO4, 0.1 g/dl sodium citrate, 0.02 g/dl MgSO4.7H2O, and 0.5 g/dl glucose. After 3 days cultivation at 37° C., two colonies appeared on the agar-medium.

Provenance & Evidence

DOI	10.6084/m9.figshare.5104873.v1
Patent number	US04591562
Source dataset	uspto-grants-1986_05
ORD ID	ord-c3807252a2b846b6b79e083ef66b1ba9
Source note	uspto-grants-1986_05