Estradiol → Androst-4-en-3-one, 17-hydroxy-, (17beta)-, mixt. with (17beta)-estra-1,3,5(10)-triene-3,17-diol

HRID 339606

Reaction equation

HRID 339606 reaction equation
[CH3:1][C@@:2]12[C@@H:18]([OH:19])[CH2:17][CH2:16][C@H:15]1[C@H:14]1[C@@H:5]([C:6]3[CH:7]=[CH:8][C:9]([OH:20])=[CH:10][C:11]=3[CH2:12][CH2:13]1)[CH2:4][CH2:3]2.[CH3:21][C@@:22]12[C@@H:30]([OH:31])[CH2:29][CH2:28][C@H:27]1[C@@H:26]1[CH2:32][CH2:33][C:34]3[C@@:40]([CH3:41])([C@H:25]1[CH2:24][CH2:23]2)[CH2:39][CH2:38][C:36](=[O:37])[CH:35]=3>O>[CH3:1][C@@:2]12[C@@H:18]([OH:19])[CH2:17][CH2:16][C@H:15]1[C@H:14]1[C@@H:5]([C:6]3[CH:7]=[CH:8][C:9]([OH:20])=[CH:10][C:11]=3[CH2:12][CH2:13]1)[CH2:4][CH2:3]2.[CH3:21][C@@:22]12[C@@H:30]([OH:31])[CH2:29][CH2:28][C@H:27]1[C@@H:26]1[CH2:32][CH2:33][C:34]3[C@@:40]([CH3:41])([C@H:25]1[CH2:24][CH2:23]2)[CH2:39][CH2:38][C:36](=[O:37])[CH:35]=3

Participants

4

Reactant 2

HCID 5757EstradiolHCID 6013Testosterone3×

Product 1

HCID 66445Androst-4-en-3-one, 17-hydroxy-, (17beta)-, mixt. with (17beta)-estra-1,3,5(10)-triene-3,17-diol

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Stock solution of estradiol and testosterone were diluted with water and mixed with Freund's incomplete adjuvant. This was mixed continuously for 1 minute and either 100 ul (E1 and E3) or 200 ul (E2 and E4) were injected subcutaneously. Estrogen or testosterone release was continuous over six days. Peak measured levels were obtained in 24 hr as shown in previous studies. Animals in the E1 and E3 groups were targeted to receive 100-300 pg/ml estrogen and groups E2 and E4 were targeted to receive 400-800 pg/ml of estrogen. Animals in T1 and T2 grouping were targeted to receive 100 pg/ml testosterone.

Workup

  1. additionmixed with Freund's incomplete adjuvant
  2. additionThis was mixed continuously for 1 minute
  3. customwere obtained in 24 hr

Sources & evidence

ORD record
ord-dc843535e72741cdaa76e661d732679e
Source dataset
uspto-grants-2014_11
Patent number
US08895541B2

Linked notes

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