Thymidine → 2-Amino-9-(2-deoxy-2-fluoro-beta-D-ribofuranosyl)-6-ethoxy-9H-purine

HRID 37076

Reaction equation

HRID 37076 reaction equation
[NH2:1][C:2]1[N:10]=[C:9]2[C:5]([NH:6][CH:7]=[N:8]2)=[C:4]([O:11][CH2:12][CH3:13])[N:3]=1.[F:14][C@@H:15]1[C@H:19]([OH:20])[C@@H:18]([CH2:21][OH:22])[O:17][C@H:16]1N1C=CC(=O)NC1=O.[N-]=[N+]=[N-].[K+].[OH-].[K+].[C@@H]1(N2C=C(C)C(=O)NC2=O)O[C@H](CO)[C@@H](O)C1>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[NH2:1][C:2]1[N:10]=[C:9]2[C:5]([N:6]=[CH:7][N:8]2[C@@H:16]2[O:17][C@H:18]([CH2:21][OH:22])[C@@H:19]([OH:20])[C@H:15]2[F:14])=[C:4]([O:11][CH2:12][CH3:13])[N:3]=1

Participants

7

Reactant 5

HCID 5789Thymidine2×HCID 14797Potassium HydroxideHCID 958056-ethoxy-9H-purin-2-amineHCID 1508512'Fluoro-2'-deoxyuridineHCID 10290740Potassium azide2×

Product 1

HCID 104257122-Amino-9-(2-deoxy-2-fluoro-beta-D-ribofuranosyl)-6-ethoxy-9H-purine

Reagents, catalysts & solvents 1

HCID 62657Potassium Phosphate, TribasicSolvent2×

Conditions

Temperature
37 °C

Procedure

2-Amino-6-ethoxypurine (0.8 g, 4.5 mmoles) which may be prepared according to R. W. Balsiger and J. A. Montgomery (J. Org. Chem. 25:1573, 1960) and 1-(2-deoxy-2-fluoro-β-D-ribofuranosyl)uracil (0.5 g, 2.1 mmoles) which may be prepared according to J. F. Codington et al. (J. Org. Chem. 29:558, 1964) were suspended in 50 ml of 5 mM potassium phosphate buffer, pH 7.0, which contained 0.04% (w/v) potassium azide. The pH of the suspension was adjusted to 7.0 with KOH. Thymidine phosphorylase (4,000 I.U.) and purine nucleoside phosphorylase (14,000 I.U.) (T. A. Krenitsky et al., Biochemistry 20:3615, 1981 and U.S. Pat. No. 4,381,344) were added and the reaction stirred at 37° C. On day 4, 2,000 I.U. of thymidine phosphorylase and 6,980 I.U. of purine nucleoside phosphorylase were added and the reaction was diluted to 250 ml with 5 mM potassium phosphate buffer, pH 7.0, which contained 0.04% (w/v) potassium azide. On day 14, the solvent was removed under vacuum. The residue was suspended in water and methanol added to precipitate the protein. After the suspension was filtered, the filtrate was evaporated. The residue was dissolved in hot water and applied to a 1.5×15 cm column of anion exchange resin (Bio-Rad AG1X2 hydroxide form). After washing the column with water, the product was eluted with methanol/water (1/1). Product-containing fractions were combined and the solvent removed under vacuum. The residue was dissolved in water and lyophilized to obtain title compound that analyzed as 0.7 hydrate.

Workup

  1. addition4,381,344) were added
  2. waitOn day 4, 2,000 I.U
  3. waitOn day 14
  4. customthe solvent was removed under vacuum
  5. additionmethanol added
  6. customto precipitate the protein
  7. filtrationAfter the suspension was filtered
  8. customthe filtrate was evaporated
  9. dissolutionThe residue was dissolved in hot water
  10. washAfter washing the column with water
  11. washthe product was eluted with methanol/water (1/1)
  12. customthe solvent removed under vacuum
  13. dissolutionThe residue was dissolved in water

Sources & evidence

ORD record
ord-35ddc882ad3142048a7706f2a15488b7
Source dataset
uspto-grants-1995_05
Patent number
US05420115

Linked notes

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