Reaction equation
[CH2:1]([OH:10])[C@@H:2]([OH:9])[CH:3]([OH:8])[C@H:4]([OH:7])[CH2:5][OH:6]>P([O-])([O-])([O-])=O>[CH2:5]([OH:6])[C@@H:4]([C@H:3]([C@@H:2]([CH2:1][OH:10])[OH:9])[OH:8])[OH:7]Participants
3
Reactant 1
Product 1
Reagents, catalysts & solvents 1
Procedure
D-arabitol was dissolved in 0.1 M phosphate buffer (pH 6.0) to a concentration of 5% (w/v). The washed microbial cells as obtained above was added thereto to about 10% (w/v) in terms of the wet weight. A 10-ml portion of the thus-obtained reaction mixture was added to a test tube and allowed to react at 30° C. with shaking. After 24 hours, the microbial cells were removed by centrifugation and xylitol thus produced was determined by HPLC. The results are shown in Table 6. As shown in Table 6, xylitol was produced and accumulated from D-arabitol.
Workup
- customThe washed microbial cells as obtained
- additionabove was added
- customA 10-ml portion of the thus-obtained reaction mixture
- additionwas added to a test tube
- customto react at 30° C.
- customthe microbial cells were removed by centrifugation and xylitol
- customthus produced
Sources & evidence
- ORD record
- ord-bf5f9a9d289f41b9b704057341fc111a
- Source dataset
- uspto-grants-2001_10
- Patent number
- US06303353B1
Linked notes
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