HuagongsheHuagongshe Chemistry Print Report · For research reference only
Phosphoenolpyruvate → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Phosphoenolpyruvate | HCID 1005 | — |
| Reactant | Potassium Chloride | HCID 4873 | — |
| Reactant | 5'-Atp | HCID 5957 | — |
| Reactant | Pyruvate | HCID 107735 | — |
| Reactant | Magnesium Chloride | HCID 5360315 | — |
| Reactant | L-Ascorbic Acid | HCID 54670067 | — |
| Solvent | 4-Morpholineethanesulfonic acid | HCID 78165 | — |
| Product | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
Reaction Steps
In 10.0 mM 2-morpholinoethanesulfonic acid (MES) buffer, 17.6 mM MgCl2, 17.6 mM KCl, 175.7 mM phosphoenolpyruvic acid (PEP), 17.6 mM ATP, 123 U/mL pyruvate kinase (PK), 97 U/mL hexokinase, and 20 U/mL ascorbic acid oxidase were dissolved in this composition, and the pH was adjusted to 7.0 to obtain a glucose converting reagent.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08753832B2 |
| Source dataset | uspto-grants-2014_06 |
| ORD ID | ord-e8dc61f2a583462c83b2582574c4ed4b |
| Source note | uspto-grants-2014_06 |