Ampicillin → (2R,3R,4S,5S)-2,3,4,5-tetrahydroxyhexanal

HRID 444671

Reaction equation

HRID 444671 reaction equation
C[C@]1(O)[C@@H]2C(=[C:12]([OH:30])[C@:13]3([OH:29])[C:20](=[O:21])[C:19](C(N)=O)=[C:18]([OH:25])[C@@H:17](N(C)C)[C@@H]3C2)C(=O)C2C(O)=CC=CC1=2.CC1(C)S[C@@H]2[C@H](NC([C@H](N)C3C=CC=CC=3)=O)C(=[O:41])N2[C@H]1C(O)=O>>[O:30]=[CH:12][C@@H:13]([C@@H:20]([C@H:19]([C@H:18]([CH3:17])[OH:25])[OH:41])[OH:21])[OH:29]

Participants

3

Reactant 2

HCID 6249AmpicillinHCID 178418840HCID 178418840, compound

Product 1

HCID 19233(2R,3R,4S,5S)-2,3,4,5-tetrahydroxyhexanal

Procedure

The plasmid pBRsaCH3 (Example 1) was used for cloning the rMET1 antibody (Example 9). In this case, the kappa and heavy chains were engineered with human ceruloplasmin and human neutrophil defensin signal sequences, respectively (Example 9). This plasmid was electroporated into E. coli strain DH10B rha+ to generate clone rMET1.LS.1, which was cultured under 10 μg/mL tetracycline selection. The plasmid was also electroporated into E. coli strain ECL339 rha− to generate clone rMET1.ECL.1 which was cultured under 100 μg/mL ampicillin selection.

Sources & evidence

ORD record
ord-215122fa05854ec2ac1d5a747ef79bea
Source dataset
uspto-grants-2013_07
Patent number
US08476040B2

Linked notes

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