MES HCl → 4-Morpholineethanesulfonic acid
Reaction equation
[CH2:1]1[N:6]([CH2:7][CH2:8][S:9]([OH:12])(=[O:11])=[O:10])[CH2:5][CH2:4][O:3][CH2:2]1.Cl>O>[CH2:5]1[N:6]([CH2:7][CH2:8][S:9]([OH:12])(=[O:11])=[O:10])[CH2:1][CH2:2][O:3][CH2:4]1Participants
3
Reactant 1
Product 1
Reagents, catalysts & solvents 1
Procedure
The heat-denatured BSA solution prepared in the above (1) (50 μl) was mixed with the sample prepared in (3) followed by addition of a MES-HCl buffer (pH 5.0) and ultrapure water to obtain a total volume of 250 μl (final concentration of MES: 50 μM) and pH was adjusted to prepare a mixed solution. A comparative mixed solution was prepared without the heat-denatured BSA solution.
Workup
- additionwas mixed with the sample
Sources & evidence
- ORD record
- ord-31b7784a7d9d46bcb3fb9a308b8fc928
- Source dataset
- uspto-grants-2013_07
- Patent number
- US08486716B2
Reaction notes
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