HuagongsheHuagongshe Chemistry Print Report · For research reference only
Udp-Glucose → 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Udp-Glucose | HCID 8629 | — |
| Reactant | Perchloric Acid | HCID 24247 | — |
| Reactant | alpha-D-glucose 1-phosphate | HCID 65533 | — |
| Reactant | Tromethamine hydrochloride | HCID 93573 | — |
| Reactant | Magnesium Chloride | HCID 5360315 | — |
| Reactant | AzddU triphosphate | HCID 131717599 | — |
| Product | 3'-Azido-2',3'-Dideoxyuridine 5-O-Diphosphoglucose | HCID 131717598 | — |
Reaction Steps
To 150 milligrams of AzddU-triphosphate was added 400 milligrams of glucose-1-phosphate, 6 millimole of MgCl2, 0.70 millimole of Tris-HCl pH 7.6, bacterial UDPG-pyrophosphorylase and inorganic pyrophosphatase. After 4 hours at room temperature, the reaction was stopped with perchloric acid (7N). After 30 minutes in ice, samples were centrifuged and the supernatant was neutralized with base. After another 30 minutes in ice, samples were recentrifuged and AzddU-5'-diphosphoglucose was purified by column chromatography.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05159067 |
| Source dataset | uspto-grants-1992_10 |
| ORD ID | ord-3cfdeae288a2409fb130096d830f42b3 |
| Source note | uspto-grants-1992_10 |