HuagongsheHuagongshe Chemistry Print Report · For research reference only
Urea → L-Tryptophan
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Urea | HCID 1176 | — |
| Reactant | Chloramphenicol | HCID 5959 | — |
| Reactant | Thiamine Hydrochloride | HCID 6202 | — |
| Reactant | Magnesium Sulfate Heptahydrate | HCID 24843 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Biotin | HCID 171548 | — |
| Reactant | Ammonium Sulfate | HCID 6097028 | — |
| Solvent | L-Threonine | HCID 6288 | — |
| Product | L-Tryptophan | HCID 6305 | — |
Reaction Steps
After culturing at 30° C. for 2 weeks, about 10,000 colonies resistant to chloramphenicol appeared, which were replicated in threonine-free medium (Thr deficient medium) which contained 2% of glucose, 1% of ammonium sulfate, 0.25% of urea, 0.1% of dihydrogen potassium phosphate, 0.04% of magnesium sulfate heptahydrate, 1 mg/dl of FeSO4.7H2O, 1 mg/dl of MnSO4.4H2O, 200 μg/dl of thiamine hydrochloride and 50 μg/dl of biotin, pH 7.0, 1.8% of agar, and 3 strains which were resistant to chloramphenicol and tryptophan independent were obtained.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US04885245 |
| Source dataset | uspto-grants-1989_12 |
| ORD ID | ord-ec4b76b4beb443f0b3ee53df3e72b8aa |
| Source note | uspto-grants-1989_12 |